摘要
目的观察电针对局灶性大脑中动脉闭塞模型(MCAO)大鼠学习记忆功能及海马CA1区白细胞介素-6(IL-6)、环氧化酶-2(COX-2)表达的影响,并探讨其可能的作用机制。方法将60只健康雄性SD大鼠随机分为假手术组(15只)和手术组(45只),手术组参照Longa线拴法制备MCAO大鼠模型,假手术组只分离相应血管,不予结扎和插入线栓。将造模后符合纳入标准的39只手术组大鼠随机分为模型组、电针组及非穴组,各13只。电针组取"百会"和"神庭"穴,非穴组取双胁下非经非穴,共电针干预7 d;假手术组和模型组在同等条件下饲养和抓取。通过神经功能评分判断大鼠的神经功能缺损情况;Morris水迷宫试验评估学习记忆能力;2,3,5-氯化三苯基四氮唑(TTC)染色检测脑梗死体积;苏木素-伊红(HE)染色观察海马CA1区神经元形态变化;免疫组化技术检测海马CA1区IL-6、COX-2的表达。结果与模型组和非穴组相比,电针组大鼠神经功能缺损评分下降,逃避潜伏期明显缩短(P<0.01),穿越平台次数增加(P<0.05),脑梗死体积明显减小(P<0.01),海马CA1区神经元损伤减轻,IL-6、COX-2的表达下降(P<0.05)。结论电针能够改善MCAO大鼠的学习记忆功能,其机制可能与下调炎症介质IL-6、COX-2在海马CA1区的表达有关。
Objective： To observe the roles of electro-acupunture on learning and memory function and the expression of IL-6 and COX-2 in hippocampal CA1 region of rats with focal cerebral ischemia/reperfusion and in- quire into its possible mechanism. Methods： 60 healthy male Sprgue-Dawley rats were randomly divided into the sham operation control group （n = 15） and the operation group （n = 45）. The focal cerebral ischemia/reperfusion model was established by suture method in the operation group. However,the sham operation control group only separated the corresponding blood vessels without ligating and inserting the suture. An amount of 39 qualified operation group rats were randomly divided into the ischemia model group （n = 13）,the electro-acupuncture group （n = 13） and the non-acupoint group （n= 13） after model establishment. For rats of the electro-acupuncture group, electro-acupuncture treatment at Baihui （DU20） and Shenting （DU24） was given for 7 days,and for rats of the non-acupoint group, electro-acupuncture stimulation was applied to bilateral costal region for 7 days. Neurological deficit scores was assessed;the ability of learning and memory was determined by Morris water maze test ;the vol- ume of the cerebral infarction Were estimated by TTC staining;the morphological changes of neurons in hippocampal CA1 region was observed by HE staining;the expression of IL-6 and COX-2 in hippocampal CA1 area was detected with immunohistochemistry technique. Results： Compared with the ischemia model group and the non-acupoint group,the neurological deficit score decreased （P〈 0.05 ）;the escape latency significantly decreased （P 〈 0.01 ） ; the times of passing through the platform increased （P 〈 0.05 ） ; the cerebral infarction volume profound- ly reduced （P〈 0.01 ）;tile pathomorphology change of hippocampal CA 1 neuron alleviated,and the expression of IL-6 and COX-2 in hippoeampal CA 1 region decreased （P〈 0.05） in the electro-acupuncture group. Conclusion ：
出处
《中国中医急症》
2016年第7期1293-1297,共5页
Journal of Emergency in Traditional Chinese Medicine
基金
福建省自然科学基金项目(2015J01335)
福建省康复技术协同创新中心资助项目(X2012004-协同)
关键词
电针
脑缺血
学习记忆
IL-6
COX-2
Electro-acupuncture
Cerebral ischemia
Learning and memory
IL-6
COX-2
