摘要
[目的]优化超声波辅助法提取柳叶蜡梅叶挥发油的工艺,并研究柳叶蜡梅叶挥发油对脂自由基的清除作用。[方法]以超声时间、提取时间、料液比3个因素进行正交试验,获得柳叶蜡梅叶挥发油的最佳提取工艺;以25%挥发油为原料、1.00 mg/ml维生素C为阳性对照研究挥发油对脂自由基的清除作用。[结果]柳叶蜡梅叶挥发油提取最佳工艺条件为超声时间2 h、提取时间8 h、料液比1∶9g/ml,得率达1.67%。挥发油对脂自由基清除作用的IC50值为208.09μl。[结论]柳叶蜡梅叶挥发油含量丰富,具有较好的脂自由基清除能力,值得进一步开发利用。
[ Objective] The ultrasonic-assisted extraction technology of volatile oil from leaf of Chimonanthus Salicifolicus Hu was investiga- ted, and the scavenging capacity against alkyl radical of volatile oil was studied. [ Method] The orthogonal experiments were carried out to study extraction technology of volatile oil with ultrasonic time, extraction time, and material-to-liquid ratio. The scavenging capacity against al- kyl radical of 25% volatile oil was studied with 1.00 mg/ml Vc as the positive control. [ Result] The optimal extraction technology was ob- tained as ultrasonic time of 2 h, extraction time of 8 h, and material-to-liquid ratio of 1：9 g/ml. Under the optimal conditions, the extraction yield of volatile oil was determined to be 1.67%. The IC5o value of scavenging capacity against alkyl radical was 208.09 μl. [ Conclusion] The volatile oil from leaf of Chimonanthus Salicifolicus Hu was rich, and its scavenging capacity against alkyl radical was good. So, it is worth to develop for application.
出处
《安徽农业科学》
CAS
2013年第4期1724-1724,1747,共2页
Journal of Anhui Agricultural Sciences
基金
黄山学院博士启动基金项目(2012xkjq002)
黄山学院大学生创新基金项目(2012xdkj004)
关键词
柳叶蜡梅
挥发油
提取工艺
脂自由基
Chimonanthus Salicifolicus Hu
Volatile oil
Extraction technology
Alkyl radical
