摘要
根据本课题组对山核桃Carya cathayensis花芽454测序获得的CcFLC基因的2个开放阅读框（ORF）分别设计引物并进行聚合酶链式反应（PCR）扩增,获得开放阅读框大小分别为639 bp和612 bp,编码212个和203个氨基酸,GenBank登录号分别为JQ829074和JQ829075,都具有典型的MADS-box结构域,核苷酸和氨基酸序列同源性分别为78%和67%,5′端高度同源,3′端差异较大。与太行花Taihangia rupestris和梨Pyrus pyrifolia等物种的FLC-like基因的氨基酸序列同源性达到40%~57%。氨基酸疏水性分析显示,它们的羧基端都是疏水性氨基酸,而氨基端是亲水性氨基酸,大部分氨基酸残基是疏水性的。实时定量PCR结果显示：CcFLC基因的2个开放阅读框在山核桃的营养枝的幼茎、幼叶、顶芽,以及短果枝的雌花芽和雄花芽中都有表达,但相对表达量存在较大差异,同一组织中ORF1的表达丰度明显高于ORF2。ORF1表达量在茎中最高,但在叶和雄花芽的表达量最低;ORF2在雌花芽中相对表达量最高,茎中表达量最低。
Based on two open reading frames （ORFs） of Carya cathayensis FLOWERING LOCUS C （CcFLC） genes which were acquired from 454 sequencings of Carya cathayensis buds, primers were designed and am- plified with polymerase chain reaction （PCR）. An amino acid hydrophobicity analysis was also conducted. Ac- quired the sequencing revealed 639 bp and 612 bp, coded 212 amino acids and 203 amino acids, and Gen- Bank accession numbers of JQ829074 and JQ829075, respectively. These had typical MADS-box structures with a nucleotide homology of 78% and an amino acid homology of 67%. The 5＇ end homology was high, but there was a big difference in the 3＇ end. Compared to amino acids coded from the FLOWERING LOCUS C （FLC）-like genes of Taihangia rupestris and Pyrus pyrifolia, the two ORFs were 40%-57% similar. Amino acid hydrophobicity analysis showed that carboxyl terminals were hydrophobic amino acid and amino terminals were hydrophilic amino acid with most amino acid residues being hydrophobic. Results of the Real-time PCR showed that the two ORFs of the CcFLC gene were expressed in the stem, young leaf, terminal bud, female flower, and male flower of C. cathayensis, but their relative expression for the same tissue was higher for ORFI than ORF2. ORF1 was maximum in the stem, but minimum in the leaf and male flower; whereas ORF2 was maximum in the female flower and minimum in the stem. [Ch, 7 fig. 23 ref.]
出处
《浙江农林大学学报》
CAS
CSCD
北大核心
2013年第1期1-8,共8页
Journal of Zhejiang A&F University
基金
国家自然科学基金资助项目(31170637)
浙江省自然科学基金资助项目(Z307534)
关键词
植物学
山核桃
CcFLC
序列分析
表达分析
botany
Carya cathayensis
CcFLC
sequence analysis
expression analysis
