摘要
目的建立通关藤药材及消癌平片中通关藤苷A的高效液相色谱含量测定方法。方法色谱柱:SinochromODS-BP分析柱(250 mm×4.6 mm,5μm);流动相:乙腈-0.1%磷酸(41∶59);检测波长:230 nm。结果通关藤苷A在0.996～19.92μg范围内与峰面积积分值呈良好的线性关系,r=0.999 95,平均加样回收率为96.6%,RSD=2.19%(n=6)。结论本方法操作简单,结果准确,稳定性和重复性好,可用于通关藤药材及消癌平片的含量测定及质量控制。
Objective To develop an HPLC method for the determination of Tenacissosides A in Maxsdenia tenacissima and Xiaoaiping tablets. Methods Sinochrom ODS-BP column （4.6 mm×250 mm, 5 μm） was used. The mobile phase consisted of acetonitrile-0.1% phosphoric acid （41 ： 59）. The UV detection wavelength was at 230 nm. Results Good linear correlation for Tenacissosides A was found in the range of 0.996-- 19.92 μg （r =0.99995）. The average recovery rate was 96.6%, with RSD of 2.19% （n =6）. Conclusion The method is simple, accurate and stable. It can be used for determination and quality control of Marsdenia tenacissirna and Xiaoaiping tablets.
出处
《中国中医药信息杂志》
CAS
CSCD
2013年第3期55-57,共3页
Chinese Journal of Information on Traditional Chinese Medicine
基金
山西省自然科学基金(2011011035-1)
