摘要
目的:探讨钯作为基体改进剂在石墨炉原子吸收光谱法测定尿铅中的应用价值。方法:用氯化钯作为基体改进剂,配制标准曲线,然后对非铅接触组和铅接触组尿液测定,用同法对尿铅标样进行测定,观察其相对误差。结果:标准1(尿比重≤1.016)的标准曲线为Y=0.746X+0.04816(r=0.9999);标准2(尿比重1.016～1.028)为Y=0.988X-0.03418(r=0.9999);标准3(尿比重>1.028)为Y=O.948X+0.05346(r=0.9999);三个标准曲线铅的最低检出浓度均为2.0μg/L。精密度实验中,平均浓度为(26.75±1.19)μg/L(、73.24±1.33)μg/L的两尿样,RSD分别为5.27%、3.47%。准确度试验中,浓度为30.26、50.26、70.26μg/L的加标尿样测得的回收率分别为102.28%、98.55%、97.62%。用该法测定尿铅标样ZK103-1(82.00μg/L)、ZK103-2(221.00μg/L)相对误差分别为1.70%、1.41%。用该法均可测定正常人及铅接触人群的尿铅浓度。结论:石墨炉原子吸收光谱法应用基体改进剂测定尿铅速度快,准确度、灵敏度高,结果可靠。
Objective： To investigate the application value about palladium as matrix modifier on determination of phpalbum in urine by GFAAS. Method： Palladium chloride as a matrix modifier, standard preparation was curved, and then the non phpalbum exposure group and phpalbum exposure group urine were determinated, and using the same method, urine phpalbum standard samples were determined, observed the relative error. Result： The standard one＇s （ urine specific gravity was less than or equal to 1.016 ） standard curve was Y=0.746X＋0.04816 （ r=0.9999 ） ; the standard 2＇s （ urine specific gravity was between 1.016 and 1.028 ） was Y=0.988X-0.03418 （ r=0.9999 ） ; the standard 3＇s （ urine specific gravity〉1.028 ） was Y=0.948X＋0.05346 （ r=0.9999 ） ; the minimum detectable concentrations of three standard curve were 2 μ g/L.In precision experiment, the RSD respectively were 5.27%, 3.47% in two urine samples whose average concentration respectively were （ 26.75 ±1.19 ） μg/L, （ 73.24 ±1.33 ）μ g/L. In accacy experiment, the recovered rate respectively were 102.28%, 98.55%, 97.62% in three standard urine samples increased amount whose concentrations respectively were 30.26, 50.26, 70.26 μ g/L.Using the same method, urinary phpalbum standard samples that were ZK103-1 （82.00μL g/L）, ZK103-2 （ 221.00 μg/L ） were determinated, and those relative errors were 1.70%, 1.41%.Using the same method, the urine lead concentration of normal persons and lead exposure persons could be determinated.Conclusion： Palladium as matrix modifier, phpalbum in urine is determinated by GFAAS, the method is speedly, its accuracy and sensitivity are high, its result is reliable.
出处
《中国医学创新》
CAS
2013年第7期91-92,共2页
Medical Innovation of China
