摘要
建立采用GB/T5009.28—2003对酒类样品进行前处理方法,用离子对高效液相法测定其中的安赛蜜和糖精钠。结果表明,检测条件:LunaC18,甲醇-2 mM四丁基氢氧化铵水溶液(用磷酸调节pH3.0)为流动相,等度洗脱,二极管阵列检测器,柱温为室温,检测波长为230 nm。糖精钠和安赛蜜线性范围为0.002～0.2 mg/mL,相关系数1,方法检出限为0.2 mg/L;在检出限范围的加标回收率分别为96.4%、99.8%;精密度分别为3.0%、2.5%。该方法的线性范围宽,精密度良好、方法检出限范围的回收率高,有良好的色谱选择性,检出限满足国标要求,适合作为国标方法的验证方法之一。
Based on wine samples pretreatment methods in GB/T5009.28-2003, a detection method by ion pair HPLC had been developed for the detection of acesulfame potassium and sodium saccharin in wine. The detection process was as follows： the separation was performed on Luna C 18 at room temperature by isocratic elution with 2 mM aqueous solution tetrabutylammium hydrogen （adjusted to pH 3 with phosphoric acid） containing 40 % methanol, and diode array detector used and the detection wavelength was 230 nm, the linear range of saccharin and acesulfame were within the concentrations of 0.002 mg/mL to 0.2 mg/mL and the correlation coefficient was 1, the detection limit was 0.2 mg / L, the recov- ery rates were 96.4 % and 99.8 % respectively, and RSD were 3.0 % and 2.5 % respectively. Such method had the advantages including wide lin- ear range, good precision, high recovery rate within the range of detection limit, and good chromatographic selectivity. It was a suitable method as one of the verification methods for national methods.
出处
《酿酒科技》
北大核心
2013年第3期104-107,共4页
Liquor-Making Science & Technology
基金
广州市科技项目基础研究专项(11C15380690)
