摘要
目的:为了探讨力竭运动引起骨骼肌损伤过程中Caveolin-3、Myogenin和MyoD的作用,以雄性Wister大鼠为研究对象,采用一次性下坡跑运动,坡度为-16°,跑速16 m/s,分别在力竭后即刻、6、12、24、48 h和4、7 d时取材。腓肠肌进行HE染色,测定血清肌酸激酶活性,RT-PCR法测定Caveolin-3、Myoge-nin、MyoD mRNA的表达。结果:骨骼肌形态学变化在损伤后24 h、48 h最严重;血清肌酸激酶在6、12、24、48 h时均显著高于对照组,其中6 h表达最高;Caveolin-3、MyoD和Myogenin mRNA均表现在先升高,后下降,其中MyoD mRNA在运动后12 h达到峰值,Caveolin-3和Myogenin mRNA在24 h达到峰值。结论:Caveolin-3、Myogenin、MyoD均参与到骨骼肌损伤及修复过程中,变化特点可能与三者在肌发生过程及肌损伤修复过程所起作用不同有关。
Objective ： to discuss the effect of Caveolin - 3, Myogenin and MyoD during the rehabilitation of skeletal injury. Male Wisters were arranged to decline in the treadmill （ - 16° slope, 16 m/s speed）. Blood and gastroene- mius was obtained immediately, 6, 12, 24, 48 h and 4 and 7days after one bout exhausting exercise. Serum CK was measured, gastrocnemius were HE dyed, Caveolin - 3, Myogenin, MyoD mRNA were determined by RT-PCR. Re- suits：The injury of gastrocnemius was the most seriously at 24 h and 48 h; Serum CK was the highest at 6 h; Caveo- lin -3, Myogenin, MyoD mRNA rose firstly and decreased lately, but MyoD mRNA reached the peak at 12 h. How- ever, Caveolin - 3 and Myogenin mRNA reached the peak at 24 h simultaneously. Conclusion ： Caveolin - 3, MyoD and Myogenin took part in the process of the rehabilitation of skeletal muscle injury, their characteristics were per- haps connected with their effects during the myogenesis and the rehabilitation of skeletal muscle injury.
出处
《北京体育大学学报》
CSSCI
北大核心
2012年第12期57-61,共5页
Journal of Beijing Sport University
基金
国家自然科学基金(编号:31040046)
辽宁省自然科学基金(编号:20102125)
辽宁省教育厅项目(编号:2009A408)
