摘要
目的 扩增日本血吸虫 2 6 k Da谷胱甘肽 S-转移酶 (Sj2 6 GST)基因片段 ,构建其重组真核表达载体 ,以研制Sj GST核酸疫苗。方法 根据已知基因序列设计一对引物 ,运用 RT- PCR技术扩增 Sj2 6 GST目的基因片段 ,将其克隆到pc DNA3质粒中 ,并经酶切、PCR鉴定 ,测序证实。结果 获得 GST- pc DNA3重组克隆。结论 :本实验获得 Sj2 6 GST重组克隆 。
Aim\ To amplify the gene of 26-Kilodalton glutathione S-transferases of Schistosoma japonicum and construct a recombinant eukaryotic expression vector.Methods\ One pair of primers was designed according to the sequence of GST.The gene fragment of Sj26GST was obtained by RT-PCR,then it was cloned into a vector pcDNA3.It was corroborated through restriction enzymes cleaving,PCR and sequencing.Results\ GST-pcDNA3 recombinant clone was constructed successfully.Conclusions\ Sj26GST is a promising candidate of vaccine for Schistosoma japonicum.GSTrecombinant clone was achieved in the experiment.It is prepared for further study for DNA vaccine. \ \
出处
《中国人兽共患病杂志》
CSCD
北大核心
2000年第6期28-30,共3页
Chinese Journal of Zoonoses
基金
中山医科大学"211"工程课题
国家教委博士点基金
中国博士后基金
