摘要
目的:建立测定杜仲中儿茶素含量的高效液相色谱方法。方法:设计正交试验对样品的前处理过程进行优化,采用反相高效液相色谱法测定杜仲中儿茶素含量,色谱柱为Agilent HC-C18(4.6 mm×250 mm,5μm);流动相乙腈-0.4%磷酸溶液(13∶87);流速1.0 mL.min-1,检测波长280 nm。结果:儿茶素在9.620~194.0 mg.L-1线性关系良好(r=0.999 3),平均加样回收率为97.38%,RSD 2.1%。结论:该法简便快速、准确性和重复性好,可用于杜仲中儿茶素的含量测定。
Objective:To establish a HPLC method for the determination of catechin in Eucommia ulmoides. Method: The orthogonal test was used to optimize the pretreatment process. RP-HPLC was adopted, on a column of Agilent HC-C18(4.6 mm×250 mm, 5 μm), using the mobile phase of acetonitrile-0.4% phosphoric acid solution (13∶87), at a flow rate of 1.0 mL·min-1; the detection wavelength was set at 280 nm. Result: Catechin was linear in the range of 9.620-194.0 μg·mL-1(r=0.999 3). The average recovery of catechin was 97.38% with RSD 2.1%. Conclusion: The method is simple, rapid and reliable with good reproduciblity, which can be used for the content determination of catechin in E. ulmoides.
出处
《中国实验方剂学杂志》
CAS
北大核心
2013年第5期117-119,共3页
Chinese Journal of Experimental Traditional Medical Formulae
基金
广西中医药大学高学历科研启动基金资助项目(G06020)