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RP-HPLC法同时测定莪术油自乳化软胶囊中6种倍半萜成分的含量 被引量:5

Simultaneous Determination of 6 Kinds of Sesquiterpenes in Zedoary Turmeric Oil Self-emulsifying Soft Capsules by RP-HPLC
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摘要 目的:建立同时测定莪术油自乳化软胶囊中6种倍半萜成分(莪术二酮、莪术醇、吉马酮、莪术烯、呋喃二烯和β-榄香烯)含量的方法。方法:采用反相高效液相色谱法。色谱柱为Agilent Eclipse XDB-C18(250mm×4.6mm,5μm),流动相为乙腈-水(梯度洗脱),流速为1.0ml/min,检测波长为210nm,柱温为30℃,进样量为20μl。结果:莪术二酮、莪术醇、吉马酮、莪术烯、呋喃二烯和β-榄香烯的质量浓度分别在17.58~131.85、2.70~20.22、9.78~73.32、40.22~301.62、11.88~89.10、3.06~22.92mg/L范围内与各自峰面积积分值呈良好的线性关系(r分别为0.9999、0.9998、0.9999、0.9999、0.9998、0.9999);平均加样回收率分别为99.8%、99.5%、100.0%、99.4%、100.1%、99.3%,RSD分别为1.27%、0.71%、0.76%、1.21%、1.07%、1.51%(n均为6)。结论:该方法简便、快速、准确、灵敏度高、重复性好、稳定可靠,可用于莪术油自乳化软胶囊的质量控制。 To establish a method for simultaneous determination of 6 kinds of sesquiterpenes in Zedoary Turmeric Oil self-emulsifying soft capsule, including curdione, curcumol, germacrone, curzerene, furanodiene and fl-elemene. METHODS: RP-HPLC method was adopted. The determination was performed on Agilent Eclipse XDB-C18(250 mmx4.6 mm, 5 pm) column with mobile phase consisted of acetonitrile-water (gradient elution) at flow rate of 1.0 ml/min. The detection wavelength was set at 210 nm and column temperature was 30 .C. The injection volume was 20 ktl. RESULTS: The linear ranges of curdione, curcumol, germacrone, curzerene, furanodiene and fl-elemene were 17.58-131.85 mg/L(r=0.999 9), 2.70-20.22 mg/L(r=0.999 8), 9.78-73.32 mg/L(r=0.999 9),40.22-301.62 mg/L(r=0.999 9), 11.88-89.10 mg/L(r=0.999 8) and 3.06-22.92 mg/L(r=0.999 9), respectively. The average recoveries were 99.8% (RSD= 1.27%, n=6), 99.5% (RSD=0.71%, n=6), 100.0% (RSD=0.76%, n= 6), 99.4% (RSD= 1.21%, n=6), 100.1% (RSD= 1.07%, n=6) and 99.3% (RSD= 1.51%, n=6), respectively. CONCLUSION: The method is simple, rapid, accurate, sensitive, reproducible, stable and reliable. It could be used for the quality control of Zedo- arv Turmeric Oil self-emulsifving, soft cangnle
出处 《中国药房》 CAS CSCD 2013年第11期1010-1012,共3页 China Pharmacy
基金 上海市科学技术委员会科研计划项目资助(No.11DZ1972500)
关键词 反相高效液相色谱法 梯度洗脱 莪术油 自乳化软胶囊 倍半萜类成分 含量测定 RP-HPLC Gradient elution Zedoary Turmeric Oil Self-emulsifying soft capsule Sesquiterpene Content deter-mination
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