摘要
对分离的纳豆激酶生产菌BSNK-5进行初步鉴定;并对其液体发酵产酶条件进行了初步研究,旨在提高纳豆激酶的产量。通过鉴定BSNK-5为枯草芽孢杆菌属菌株。试验优化了发酵培养基的最适氮源和碳源种类及百分含量,发酵培养基的起始pH值,最适种子接种量,发酵温度,培养时间等。最终确定了两个最适的产酶条件,在该条件下,BSNK-5通过液体发酵产纳豆激酶的活力高达1 160 U/mL,比优化前提高283%。
Nattokinase production bacteria BSNK-5 was prefiminarily identified. The effects of liquid fermentation on nattokinase activity produced by BSNK-5 were studied in order to improve production yield. BSNK-5 is a Bacillus subtilis. Different carbon sources, nitrogen sources, pH, inoculate size;temperature, and time were optimized. Two optimum culture conditions were obtained. The nattokinase activity was 1 160 U/mE under the optimum culture conditions, which was 283% times of the former culture conditions.
出处
《生物技术通报》
CAS
CSCD
北大核心
2013年第2期184-189,共6页
Biotechnology Bulletin
基金
中国农业科学院科技经费项目(201211)
