摘要
目的检测乙型肝炎病毒患者乙型肝炎病毒前-S1抗原(HBV pre-S1-Ag)、前-S2抗原(HBV pre-S2-Ag)、乙型肝炎病毒DNA(HBV DNA)和乙型肝炎病毒E抗原(HBeAg),探讨其相关性和临床应用价值。方法应用酶联免疫测定法(ELISA)分别检测HBV pre-S1-Ag、HBV pre-S2-Ag和乙型肝炎病毒标志物(HBV-M),荧光定量聚合酶链反应法(FQ-PCR)检测HBV DNA,并对检测结果进行统计学分析。结果 HBsAg阳性者中,pre-S1-Ag、pre-S2-Ag、HBV DNA阳性者分别为594例、541例、629例,其阳性率分别为66.29%、60.38%、70.20%。HBeAg阳性组pre-S1-Ag、pre-S2-Ag、HBV DNA的阳性率分别为90.21%、74.46%、93.32%,显著高于HBeAg阴性组的45.28%、48.01%、49.89%,差异有统计学意义(P<0.01)。随着HBV DNA载量的增高,pre-S1-Ag、pre-S2-Ag、HBeAg阳性率随之增高。结论 pre-S1-Ag、pre-S2-Ag与HBV DNA和HBeAg阳性检出率具有显著相关性。联合检测pre-S1-Ag、pre-S2-Ag、HBV DNA及HBV-M,有助于HBV早期诊断、疗效观察和预后判断。
Objective To detect pre-S1 antigen（HBV pre-S1-Ag）,pre-S2 antigen（HBV pre-S2-Ag）,hepatitis B virus（HBV） DNA and hepatitis B e antigen（HBeAg） in hepatitis B patients and discuss the correlation among them and the clinical applications value.Methods Enzyme-liked immunosorbent adsorption method（ELISA） were applied to detect HBVpre-S1-Ag,HBV pre-S2-Ag and markers of hepatitis B virus（HBV-M）.Fluorescence quantitative polymerase couplet reaction method（FQ-PCR） was applied to detect HBV DNA and the detection results were analyzed statistically.Results Among the HBsAg positive patients,the pre-S1 antigen,pre-S2 antigen,HBV DNA positive patients were 594 cases,541 cases,629 cases,respectively,with the rate of 66.29%,60.38%,70.20%.HBeAg positive patients＇ pre-S1 antigens,pre-S2 antigens,HBV DNA positie rates were 90.21%,74.46%,93.32%,signif icantly higher than that in HBeAg negative patients with 45.28%,48.01% and 49.89%,respectively.The difference were statistically signif icant（P 0.01）.Pre-S1-Ag,pre-S2-Ag and HBeAg positive rates arised with HBV DNA arising.Conclusions Pre-S1-Ag,pre-S2-Ag and HBV DNA and HBeAg positive rate correlate signif icantly.United detection of pre-S1-Ag,pre-S2-Ag,HBV DNA and HBV-M may contribute to hepatitis B early diagnosis and clinical observation and prognosis.
出处
《中国肝脏病杂志（电子版）》
CAS
2011年第1期27-30,共4页
Chinese Journal of Liver Diseases：Electronic Version
