摘要
应用PCR及PAGE电泳技术对武汉汉族人群作DIS1656、D2S441、DHFRP2、D8S1132、D10S2325、DXS6804等STR位点进行遗传学多态性调查,结果DIS1656、D2S441、DHFRP2、D8S1132、D10S2325位点分别检出基因10,8,5,10,11个,分别检出基因型42、26、13、38、44个;DXS6804女性检出5个等位基因型,14个基因型,男性检出7个等位基因.经检验,6个STR基因座基因型分布均符合Hardy-Weinberg平衡,累积个人识别能力为0.99999987,累积非父排除率为0.9939.DIS1656等6个STR均属高杂合度,高鉴别能力的遗传标记系统,在法医学个人识别和亲子鉴定中有较高实用价值.
Allele frequency distributions and the genetic polymorphism of six STR loci(DlS1656、D2S441、DHFRP2、D8S1132、D10S2325、DXS6804)were studied from Han population living in Wuhan, China, the PCR products were analyzed by PAGE, followed by silver stains, self - made allelic ladder was used as stand and size marker. lOalleles and 42 gene type of D1S1656; 8 alleles and 26 gene types of D2S441; 5 alleles and 13 gene types of DHFRP2; 10 alleles and 38 gene types of D8S1132; 11 alleles and 44 gene types of D10S2325 were detected from 200 unrealitive individuals. The distribution of gene type in six loci are good agreement with Hardy - Weinberg equilibrium. The total Dp of six loci is 0. 99999987. The total Epp of six loci is 0. 9939. Our results show these six STR loci are valuable STR genetic marker system having high heterozyosity and Dp. These can be used in forensic individualization and paternity test.
出处
《刑事技术》
北大核心
2000年第1期5-8,共4页
Forensic Science and Technology
基金
本课题为公安部重点资助项目
关键词
短串联重复序列
STR
基因
汉族人
法医学
STR, polymorphism, PCR, allele frequency, individual identification
