摘要
目的研究宁夏回族群体X染色体上的10个短串联重复序列(DXS101,DXS6789,DXS6799,DXS6804,DXS7130,DXS7132,DXS7133,DXS7423,HPRTB,DXS8378)的基因多态性。方法随机抽取100名宁夏回族无关个体(男性40名,女性58名)的静脉血,提取DNA,PCR扩增,变性聚丙烯酰胺凝胶电泳,银染检测结果。结果DXS101、DXS6789、DXS6799、DXS6804、DXS7130、DXS7132、DXS7133、DXS7423、HPRTB、DXS8378分别检出9、8、4、6、6、6、4、4、5和5十种等位基因,检出17、22、7、14、14、15、6、7、12和8十种基因型;10个位点等位基因频率分别分布在0.0087-0.3130、0.0087-0.2696、0.0348-0.5826、0.0087-0.3044、0.0261-0.4348、0.0261-0.3217、0.0261-0.6783、0.0087-0.4870、0.0261-0.4783和0.0087-0.4870之间;此10个位点女性的基因型频率分布均符合Hardy-Weinberg平衡。多态信息量除DXS7133和DXS7423外均大于0.50;女性个体识别力从0.89(DXS7133,DXS7423)至0.99(DXS101,DXS6789,DXS7132)。结论此10个X染色体STR位点有较高的个体识别率,在个体识别和女孩亲权鉴定中有较高应用价值,对疾病相关研究有重要意义。
Objective To investigate the genetic polymorphism of 10 short tandem repeat （STR） loci on the X chromosome（DXS101, DXS6789, DXS6799, DXS6804, DXS7130, DXS7132, DXS7133, DXS7423, HPRTB, DXS8378） of Chinese Hui population. Methods The 10 X-chromosomal STR loci were performed by PCR following polyacylamide gel electrophoresis and silver stain. Results DNA extracts were obtained from unrelated Hui individuals; the number of alleles in the 10 X-STR loci DXS101, DXS6789, DXS6799, DXS6804, DXST130, DXS7132, DXS7133, DXS7423, HPRTB and DXS8378 was 9, 8, 4, 6, 6, 6, 4, 4, 5 and 5, respectively the number of genotypes was 17, 22, 7, 14, 14, 15, 6, 7, 12 and 8, respectively. The distribution of genotypes of these 10 STR systems fit the Hardy-Weinberg equilibrium （P〉0.05）. The allele frequency of these 10 loci were 0. 008 7- 0.3130, 0.008 7-0.269 6, 0.034 8-0.582 6, 0.008 7-0.304 4, 0.026 1-0.434 8, 0.026 1-0.321 7, 0.026 1- 0.678 3, 0.008 7-0.4870, 0.026 1-0.4783 and 0.008 7-0.4870. Polymorphisms information content of 10 STR loci, except for DXS7133（0.48） and DXS7423（0.48）, ranged from 0.54（DXS6799） to 0.80（DXS6789） ; the PD^（F） were from 0.89 （DXS7133, DXS7423） to 0.99 （DXS6789, DXS7132, DXS101）. Conclusion The 10 X-chromosomal STR loci are appropriate for individual identification and paternity testing of a female child as well as for studies on related diseases.
出处
《西安交通大学学报（医学版）》
CAS
CSCD
北大核心
2006年第5期441-444,共4页
Journal of Xi’an Jiaotong University（Medical Sciences）
关键词
X染色体
短串联重复
遗传多态性
回族
x-chromosome
short tandem repeat（STR）
genetic polymorphism
