摘要
目的:观察化瘀祛痰方药及其拆方对THP-1源性泡沫细胞腺苷三磷酸结合盒转运体A1(ABCA1)、CD36表达的影响,探讨该方药的作用机制,并比较方药全方及各拆方的疗效及调控作用。方法:体外培养THP-1细胞,佛波酯(PMA)使之巨噬化,氧化型低密度脂蛋白(ox-LDL)使巨噬细胞泡沫化。细胞随机分为空白对照组、模型组、补气组、化瘀组、祛痰组和全方组。油红O染色观察细胞浆脂滴变化,酶法测定细胞内总胆固醇(TC)、游离胆固醇(FC)、胆固醇酯(CE)的含量,并计算CE/TC值。反转录-聚合酶链反应(RT-PCR)定量分析ABCA1、CD36 mRNA水平。结果:模型组细胞TC、FC、CE含量及CE/TC值均显著高于对照组(P<0.01);与模型组相比,全方组和祛痰组细胞TC、FC、CE含量及CE/TC值均显著降低。RT-PCR结果提示模型组细胞ABCA1、CD36 mRNA水平显著升高;而化瘀祛痰方药及祛痰拆方含药血清可显著升高ABCA1 mRNA,显著降低CD36 mRNA水平。结论:化瘀祛痰方药及祛痰方可抑制ox-LDL诱导的THP-1细胞泡沫化,该作用可能与其上调ABCA1基因、下调CD36基因表达,从而减弱THP-1细胞对ox-LDL摄取有关。
Objective：To observe the influence of Huayu Qutan recipe and its separated recipes on the expressions of ABCA1 and CD36 in macrophage-derived foam cell model of THP - 1 cell line. Methods：Human THP - 1 cells were cultured with 160 nmol/L PMA for 24 h in order to differentiate the cells to macrophages. The macrophages were incubated with 100 mg/L ox - LDL for 24 h to form foam cells. Then the cells were equally divided into six groups randomly;the normal serum control, model, Buqi recipes ,Huayu recipes, Qutan recipe groups and Huayu Qutan recipes groups. RT -PCR method was used to test the changes of expressions of ABCA1 and CD36. Results：In model group ,the contents of TC, FC, CE, CE/TC ratio as well as the expressions of ABCA1 and CD36 were significantly increased compared with control group. Compared with model group, the contents of TC, FC, CE,CE/TC ratio and expression of ABCA1 were obviously increased while the expression of CD36 was obviously decreased in the Huayu Qutan recipe group and Qutan recipe group. Conclusion ：Huayu Qutan recipe and Qutan recipe could inhibit atheroselerotic foam formation through up -regulating ABCA1 expression and down -regulating CD36 expression.
出处
《辽宁中医杂志》
CAS
2013年第2期234-237,共4页
Liaoning Journal of Traditional Chinese Medicine
基金
国家自然科学基金青年基金项目(81202834)
辽宁省高等学校杰出青年学者成长计划资助项目(LJQ2011100)
中国博士后科学基金特别资助项目(201104611)
