摘要
[Objective] The paper was to ascertain the fermentation conditions and medium formula of Bacillus subtilis B-332. [Method] Using single factor and orthogo- nal experimental designs, the fermentation medium and culture conditions for B. sub- i tills B-332 were optimized through shaking flask culture. [Result] The optimal culture and inoculation time was 18 h, and the optimized medium formula was as follows： soybean powder 0.60 g/L, sucrose 0.25 g/L, ammonium sulfate 0.07 g/L, trisodium citrate 0.03 g/L, dipotassium hydrogen phosphate 0.003 g/L, magnesium sulfate 0.005 g/L, ferrous sulfate 0.000 5%; the fermentation condition was as follows： tem- perature 30 ℃, shaking bed speed 180 r/min, shaking bottle volume 80 ml/500 ml. In the optimal fermentation conditions, the fermented spore quantity was 1.43×10^11 cfu/ml, which was 34.48 times higher than the spore quantity of 4.03×10^9 cfu/ml in initial condition. [Result] The study laid the foundation for factory production of the strain.
[目的]探明生防菌株枯草芽孢杆菌B-332的发酵条件及培养基配方。[方法]采用单因素和正交试验设计,通过摇瓶培养对枯草芽孢杆菌B-332菌株的发酵培养基和培养条件进行优化。[结果]最适培养接种时间为18h,优化后的培养基配方为:豆饼粉0.60g/L、蔗糖0.25g/L、硫酸铵0.07g/L、柠檬酸三钠0.03g/L、磷酸氢二钾0.003g/L、硫酸镁0.005g/L、硫酸亚铁0.0005g/L;发酵条件为:温度30℃、摇床转速180r/min、摇瓶装量80ml/500ml。在该综合条件下,发酵后的芽孢数为1.43×1011cfu/ml,比初始条件的芽孢总数4.03×109cfu/ml提高了34.48倍。[结论]该研究为枯草芽孢杆菌B-332菌株的工厂化打下了基础。
基金
Supported by Major Science and Technology Project of National Water Pollution Control and Management "Pollution Control against Agricultural Non-point Source Pollution into River and Ecological Restoration Technology and Engineering Demonstration Project"(2009ZX07103-002)
"the Eleventh Five-Year" National Key Technology Support Program "Control Technology and Demonstration of Overall Planning of Urban and Rural Life Rubbish Pollution" (2009BADC2B00-02)~~
