摘要
目的了解本院近两年来血液感染病原菌的种类及其对常用抗生素的敏感情况,指导临床合理使用抗生素。方法采用Bactec 9120细菌培养仪进行血液标本的培养,VETEK-Ⅱ细菌鉴定仪和phoenix-100全自动细菌分析仪进行细菌鉴定及药敏试验,并应用WHONET5.4软件进行数据分析。结果临床送检的8 040份血培养标本中,分离出病原菌898株,阳性率11.2%。最常见的主要为大肠埃希菌(164株,占18.3%)、表皮葡萄球菌(112株,占12.5%)和肺炎克雷伯菌(83株,占9.2%);革兰阴性菌敏感性最好的药物为亚胺培南,其次为哌拉西林/他唑巴坦、头孢哌酮/舒巴坦,革兰阳性球菌敏感性最好的药物为替考拉宁和万古霉素。结论近两年本院血液感染致病菌以革兰阴性菌为主,且菌种多样化,有较高耐药率,提示应定期对血液感染病原菌的分布和耐药情况进行监测,以便及时了解病原菌变化及耐药趋势,监督指导临床用药。
Objective To investigate the changes of bacterial spectra and drug resistance of pathogenic bacteria in blood culture,and provide a basis for clinical treatment.Methods Blood was cultured by Bactec 9120 of BD.The clinical isolates were identified by VETEK-Ⅱand phoenix-100 of BD.The results was analyzed by WHONET5.4.Results 898 strains of pathogenic bacteria were isolated from 8 040 clinic blood specimens,of which Escherichia coli（164 strains,18.3%）,Staphylococcus epidermidis（112 strains,12.5%） and Klebsiella pneumoniae（83strains,9.2%） are most familiar.The sensitivity of Gram-negative bacteria to imipenem was the highest;the sensitivity of Gram positive bacteria to teicoplanin and vancomycin were the highest.Conclusion Gram-negative bacteria are the main stains in blood culture in the hospital,and they are diverse.The rate of the drug resistance of some bacteria is high,which indicated that it is necessary to analyze the resistance of pathogens and its distribution regularly.Monitoring the changes of pathogens and trends of drug resistance is very important in guiding the clinical use of antibiotics.
出处
《黑龙江医学》
2012年第11期831-833,共3页
Heilongjiang Medical Journal
关键词
血培养
菌株
耐药性
Blood culture
Pathogen
Resistance
