摘要
目的:研究贵州省6个世居少数民族线粒体9bp序列缺失情况。方法:采用PCR法及聚丙烯酰胺凝胶技术检测线粒体基因组色素氧化酶Ⅱ和tRNAlys基因间非编码序列中含9bp序列缺失情况。结果:在研究的6个少数民族中只发现标准型和缺失性2种多态性,仡佬族、仫佬族、畲族、毛南族、羌族、蒙古族线粒体DNA 9bp缺失频率依次为:26.9%、17.3%、23.1%、11.5%、7.7%、9.6%。结论:贵州省这6个世居少数民族线粒体9bp序列缺失频率较高,各民族的缺失频率有一定差异。
Objective：To analyze the frequency of 9bp deletion of mitochondrial DNA in 6 ethnic groups of Guizhou. Method：Polymerase chain reaction was adopted to amplify the mtDNA segment between the cytochrome oxidase Ⅱ and tRNAlys containing the 9bp deletion and polyacrylamide slab gel electrophoresis was used to detect PCR products. Result：The frequency of 9bp deletion of mitochondrial DNA in Gelao, M ulao, She, Maonan, Qiang, Mernggu people was 26. 9%, 17. 3 %, 23. 1%, 11.5 %, 7. 7 %, 9. 6 %. Conclusion： The frequency of 9bp deletion of mitochondriai DNA in 6 ethnic groups of Guizhou was relatively high,there were some differences among these groups.
出处
《生物技术》
CAS
CSCD
北大核心
2012年第5期51-54,共4页
Biotechnology
基金
贵州省科技计划项目(黔科合J[2011]2119
黔科合SY[2010]3001
黔科合LG[2011]024)资助
