摘要
目的探讨^18F—FLTPET显像能否预测肿瘤放射敏感性。方法对乳腺癌MDA—MB．231细胞和脑胶质瘤LN229细胞以6MVX线照射0、8和16Gy后分别行克隆形成细胞存活率实验和^18F—FIJT细胞摄取实验，各剂量组2种细胞的数据差异行t检验，同种细胞放疗前（0Cy）与放疗后数据差异行单因素方差分析。将MDA-MB-237和LN229细胞分别接种至雌性BALB／cnu／nu裸鼠右后肢外侧皮下，待肿瘤直径长至10mm时，将2种荷瘤鼠均按0、8和16Gy分组（随机数字表法），每组20只。各组按相应剂量放疗后分别行^18F—FLTPET显像和免疫组织化学检测，计算肿瘤与肌肉的SUV比值（T／M）和TKl标记指数（uTKl），并行相关性分析。结果8Gy辐照后MDA—MB-231细胞和LN229细胞生存分数分别为（59．73±4．3）％和（93．41±3．75）％（t=-13．20，P〈0．001），16Gy时两者生存分数分别为（43．57±4．06）％和（81．77±4．42）％（t=-14．24，P〈n001）。8Gy辐照后1hMDA—MB-231细胞18F—FLT摄取降至（18．32±1．38）kBq／10’细胞[0Cy时为（128．22±8．24）kBq／10细胞，F=266．41，P〈n01]，72h内保持在较低水平；LN229细胞辐照后1h摄取降至（9．87±1．30）kBq／105细胞[0Gy时为（134．88±6．59）kBq／10^5细胞，F：346．06，P〈0．01]，后逐渐增加，72h时升至（127．17±9．08）kBq／10细胞（F=346．06，P〉0．05）；16Gy时2种细胞摄取变化基本同8Cy组。8Gy放疗后裸鼠MDA—MB-231移植瘤18F—FlJrr摄取在第1天T／M比值下降至0．78±0．39（放疗前为2．84±0．29，F=39．78，P〈0．01），后缓慢升高，在第7天时仍低于放疗前水平（F：39．78，P〈0．01）；16Cy时变化基本同8Gy组。LN229移植瘤8Gy放疗在第1天T／M比值升高至2．41±0．47（放疗前为1．58±0．29，F=34．01，P〈0．05），后逐渐降低，到第7天降至0．66±0．32（F=34．01，P〈0．05）；16Gy放疗后^18F—FLT摄取值持续下降
Objective To evaluate if lSF-FLT PET imaging could be used as a new clinical method to predict tumor radiosensitivity. Methods MDA-MB-231 and LN229 ceils were irradiated with doses of 0, 8 and 16 Gy of 6 MV photon energy, then soft agar assay and cellular uptake of 18F-FLT were performed on the 2 eel1 lines. The t test and one-way analysis of variance were used for the two groups and data beforeand after irradiation. The MDA-MB-231 and LN229 tumor xenografts were prepared by injecting the tumor cells into the right limbs of female BALB/c nu/nu mice. Once tumors reached a diameter of 10 mm, the two types of mice were divided randomly into 3 groups （20 mice per group） according to the irradiation doses （0, 8 and 16 Gy）. After irradiation, is F-FLT PET imaging and immunohistochemical staining were conduc- ted. Then correlations between is F-FLT SUVtumor/SUVmuscle ratio （T/M ratio） and TK1 labeling index percentages （ LITK1 ） were tested using linear correlation analysis. Results The survival fraction of MDA-MB- 231 and LN229 cells after irradiated with 8 Gy were （59.73 ± 4.3 ） % and （93.41 ± 3.75 ） %, respectively （t = - 13.20, P 〈0. 001 ）. When the dose increased to 16 Gy, the survival fraction decreased to （43.57 ± g. 06） % and （81.77 ± 4.42） %, respectively （t = - 14.24, P 〈 0. 001 ）. In MDA-MB-231 cells, the cel- lular uptake of ]8F-FLT after irradiation with 8 Gy declined rapidly to （ 18.32 ± 1.38 ） kBq/105 cells （ （ 128.22 ± 8.24） kBq/105 cells with the dose of 0 Gy, F = 266.41, P 〈 0.01 ）, and maintained this low level till 72 h. For the LN229 cells, the cellular uptake decreased to （9.87 ± 1.30） kBq/105 cells after 8 Gy irradiation （ （ 134.88 ± 6.59） kBq/105 cells with the dose of 0 Gy, F = 346.06, P 〈 0. O1 ）, then increased gradually to （ 127.17 ± 9.08） kBq/105 cells at 72 h （ F = 346.06, P 〉 0.05）. The dynamic changes of lSF-FLT cellular uptake in the two cells had the same pattern after being treated with 16 Gy irr
出处
《中华核医学与分子影像杂志》
CSCD
北大核心
2013年第1期60-64,共5页
Chinese Journal of Nuclear Medicine and Molecular Imaging
基金
天津市卫生局科技基金（09KZ83）
