摘要
目的:观察鞘内注射高浓度医用臭氧对大鼠运动功能和脊髓组织中Bax、Bcl-2基因表达的影响。方法:SD大鼠16只,随机均分为2组(n=8),分别建立大鼠鞘内置管模型。对照组(C组)仅行鞘内穿刺置管不注射;医用臭氧组(O3-60组):鞘内置管后经导管注射60μg/mL医用臭氧10μL。麻醉前及注射医用臭氧后24 h分别对两组大鼠进行BBB运动功能评分;然后立即断颈取材,采用Western blot测定腰膨大处脊髓组织Bax、Bcl-2蛋白表达水平。结果:与对照组相比,鞘内注射高浓度医用臭氧24 h后,臭氧组大鼠后肢BBB运动功能评分(Basso Beattie andBresnahan locomotor rating scales,BBB scores)显著降低(P<0.05);脊髓Bax蛋白表达显著增加(P<0.05),Bcl-2/Bax比值显著降低(P<0.05)。结论:高浓度医用臭氧鞘内注射可损伤大鼠运动功能,其损伤可能与神经细胞凋亡有关。
Objective： To investigate the effects of intrathecal administration of high concentration of ozone on the animal behavior and protein expression of Bax and Bcl-2 in spinal cord of rats. Methods： Sixteen healthy adult Sprague-Dawley rats were randomly divided into two groups （n = 8）： the control group （Group C） and the 60μg/mL 03 group （Group O3-60i. In Group C, rats were intrathecally catheterized without any medication, while in the Group 03-60, 60 μg/ml oxygen-ozone 10 μL was administered intrathecally. The BBB locomotor rating scales （the BBB score） were recorded before anesthesia and 24h after 03-60 administered. The lumbar spinal cord was collected to detect the protein expression of Bax and Bcl-2 by western blot. Results： After intrathecal 03-60 administration, compared with Group C, the BBB score of Group 03-60 was significantly lower （P 〈 0.05）. The expression of Bax was significantly higher （P 〈 0.05）, and the ratio of Bax/Bcl-2 of Group 03-60 was significantly lower （P 〈 0.05）. Conclusion： The intrathecal administration of high concentration of ozone might damage the behavior of rats, and the neurotoxicity of ozone may be related to the procession of neuron apoptosis.
出处
《中国疼痛医学杂志》
CAS
CSCD
北大核心
2013年第2期95-97,共3页
Chinese Journal of Pain Medicine
基金
国家自然科学基金资助项目(30972844、81271346)
山东省自然科学基金资助项目(Y2006C69)
