摘要
目的检测Trib1同源基因rs17321515位点基因型多态性分布状况,并探索该位点多态性与不同血脂指标和血脂临床诊断结果的关系。方法采集1 014名体检者的血样,进行血脂四项指标测定,并采用MALDI-TOF质谱技术对rs17321515位点基因型进行分型检测。结果 rs17321515位点基因型构成比为:A/A∶A/G∶G/G=13.8%∶50.2%∶36.0%,等位基因频率为A∶G=38.9%∶61.1%。方差分析中,男性不同基因型的TG水平存在统计学差异(F=4.46,P=0.01),AG携带者比GG携带者的TG水平低(t=-0.29,P=0.02);Logistic回归分析表明,男性AA型携带者高TG血症患病率低于GG型(OR=0.45,P=0.04),男性AG型携带者低HDL-c血症患病率低于GG型携带者(OR=0.62,P=0.03),女性AG型携带者高TC血症患病率低于GG型携带者(OR=0.58,P=0.01)。结论 rs17321515位点等位基因频率在不同种族人群存在一定差异;该位点多态性对多个血脂指标和血脂异常临床诊断都有影响,但这种影响存在性别差异;A等位基因是血脂水平的保护因素。
Objective To study on genotype distribution of rs17321515 which is homologous with Tribl and its associativity with blood lipid parameters and diagnosis. Method Collecting 1 014 blood samples to measure its lipid levels, then detecting rs17321515 locus＇s SNP by MALDI-TOF mass spectrometry technology. Results three genotypes（ A/A, A/G, G/G） proportions in the population were 13.8% , 50.2% and 36.0% ,allele frequencies were A： G = 38.9% ： 61.1%. In variance analysis, variation of TG of male was statistical significant（ F = 4.46, P = 0.01 ） , TG level of male who carriedAG is lower than GG carries （ t = 0.29, P = 0.02 ）. Logistic regression analysis showed that,hypertriglyceridemia prevalence of male who carried AA is lower than GG carries （ OR = 0.45,P = 0.04）. Low HDL-c acidosis prevalence of male who carried AG is lower than GG carries（ OR = 0.62, P = 0.03 ）. Hypercholesterolemia prevalence of female who carried AG is lower than GG carries （ OR = 0.58, P = 0.01 ）. Conclusion Allele frequencies of rs17321515 varies in different ethnic groups; The locus＇ polymorphism distribution is relevant with a certain lipid indicators and lipid diagnosis, but there exist gender differences on these relevance.
出处
《卫生研究》
CAS
CSCD
北大核心
2013年第1期82-86,共5页
Journal of Hygiene Research
基金
杭州市科技发展计划项目(No.20090233T07)
