摘要
目的研究TRIB1基因内含子区rs2235109多态性与冠心病的关系及其在湖北省汉族人群中的分布特征,并比较其基因型和等位基因在正常人和冠心病人群中的分布情况。方法采用聚合酶链反应-限制性片段长度多态(PCR-RFLP)方法对湖北地区152名健康汉族人和42名冠心病患者的TRIB1基因单核苷酸多态性(single nucleotide polymorphism,SNP)进行分析。结果上述多态性位点在冠心病和正常人群中的分布无统计学差异,但具有中等多态信息量,几乎达到其PIC的理论最大值。结论 rs2235109 SNP的变异与冠心病人群无相关性,但该位点与其它SNP构成单倍型联用可作为一个理想的遗传标记。
Objective To study the relationship between TRIB1 gene intron rs2235109 polymorphism and coronary heart disease and its distribution characteristics of Han population in Hubei province,and to compare the distribution of its genotypes and alleles in normal population and coronary heart disease. Methods Polymerase chain reaction-restriction fragment length polymorphism（PCR-RFLP）method was adopted to analysis TRIB1 gene single nucleotide polymorphism of 152 healthy Han Chinese and 42 patients with coronary heart disease in Hubei province.Results The polymorphic distribution was not obviously different between the coronary heart disease and normal pollution.But it had the moderate polymorphism information content and almostly reached the theoretical maximum of PIC.
出处
《咸宁学院学报（医学版）》
2010年第5期385-388,共4页
Journal of Xianning Univarsity(medical Sciences)
关键词
TRIB1基因
单核苷酸多态性
冠心病
TRIB1 gene
Single nucleotide polymorphisms
Coronary heart disease
