摘要
本试验旨在研究培养基中添加不同浓度的胰岛素对奶牛乳腺上皮细胞生长及κ-酪蛋白(CSN3)和胰岛素受体(INSR)基因表达的影响。选用中国荷斯坦奶牛的乳腺上皮细胞进行体外培养,在以无血清无激素的生长培养基为对照的基础上,分别添加胰岛素5、50、500和5 000 ng/mL,测定CSN3和INSR基因表达量以及CSN3相对含量的变化。结果表明:1)胰岛素能够促进乳腺上皮细胞的增殖,其中5～500 ng/mL的胰岛素促增殖作用较好。2)胰岛素能提高乳腺上皮细胞CSN3、INSR基因表达量和CSN3相对含量,50 ng/mL组CSN3基因表达量和CSN3相对含量均显著或极显著高于对照组(P<0.01)以及5(P<0.01)、500(P<0.01)和5 000 ng/mL组(P<0.05),INSR基因表达量显著或极显著高于对照组(P<0.05)以及500(P<0.05)和5 000 ng/mL组(P<0.01)。结果提示,随着胰岛素浓度的增加,CSN3和INSR基因的表达量及CSN3相对含量均呈先增加后下降的趋势,50 ng/mL时最高,而高浓度(5 000 ng/mL)的胰岛素不利于奶牛乳腺上皮细胞合成乳蛋白。
This study was conducted to investigate the effects of insulin on growth and expressions of κ-casein（CSN3） and insulin receptor（INSR） genes in bovine mammary epithelial cells.Bovine mammary epithelial cells from Chinese Holstein were used in an in vitro cultivation.Cells were cultured in a growth culture medium（without serum and hormone,control group）,and the growth culture medium supplemented with 5,50,500 and 5 000 ng/mL insulin,respectively.Changes of expression levels of CSN3 and INSR genes and relative content of CSN3 were determined.The results showed as follows： 1） insulin could promote the proliferation of bovine mammary epithelial cells,and the optimal level was 5 to 500 ng/mL.2） The expression levels of CSN3 and INSR genes and relative content of CSN3 were increased by insulin,CSN3 gene expression level and CSN3 relative content in 50 ng/mL group were significantly higher than those in control group（P0.01） as well as 5（P0.01）,500（P0.01） and 5 000 ng/mL groups（P0.05）,and INSR gene expression level was significantly higher than that in control group（P0.05） as well as 500（P0.05） and 5 000 ng/mL groups（P0.01）.The results indicate that with the increase of insulin concentration,expression levels of CSN3 and INSR genes and relative content of CSN3 tend to be increased at first and then decreased,and the peak appears at 50 ng/mL,while high concentration of insulin（5 000 ng/mL） is unfavourable for the synthesis of milk protein in bovine mammary epithelial cells.
出处
《动物营养学报》
CAS
CSCD
北大核心
2013年第1期77-87,共11页
CHINESE JOURNAL OF ANIMAL NUTRITION
基金
国家重点基础研究发展规划(973计划)项目(2011CB100803)
江苏高校优势学科建设工程资助项目
江苏省研究生创新工程项目(CXZZ11_0989)
