摘要
目的 探讨精氨酸对高温下血清一氧化氮 (NO)含量和淋巴细胞增殖活性的影响。方法 BALB/C小鼠 ,随机分为对照组、精氨酸补充组 [0 5 ,1 5 ,2 5 ,3 5 g/ (kg .bw) ],每组 35只 ,其中 5只为常温对照。 14d后 ,除常温对照外均放入 (41± 0 5 )℃动物高温仓热应激 12 0min ,结束后 0 ,2 ,4 ,8,12 ,2 4h共 6个时相分别眼眶采血 ,常温组在灌胃第 3,7,11,14d时采尾血。观察指标测定血清NO浓度、淋巴细胞增殖活性。结果 (1)在常温下补充精氨酸后第 3d各实验组血清NO含量最高 ,第 7d后基本稳定 ,补充组各个时间点都高于未补充组 ,其中补充精氨酸 0 5 g/(kg .bw)组各个时间点明显高于其他补充组。 (2 )热应激后补充 0 5和 1 5 g/ (kg .bw)及未补充组血清NO浓度有先下降 (0～ 2h最低 )而后上升 ,4～ 8h基本稳定。而补充高浓度精氨酸的两组〔2 5和 3 5 g/ (kg .bw)〕热应激后持续上升 ,而且在 12h内 ,血清NO含量明显高于低浓度的两组和对照组。 (3)在热应激下补充精氨酸的各组刀豆蛋白(ConA)刺激指数高于对照组 ,补充精氨酸的各组 ,以 1 5 g/ (kg .bw)时ConA刺激指数达到最大。热应激后 ,小鼠淋巴细胞增殖活性受到抑制 ,其中 4～ 8h最低。结论 高温环境应激下小鼠对精氨酸的需要量增加 ,而血清NO含量的变?
Objective To discuss the effect of supplement L-arginine on concentration of nitric oxide(NO)in serum and lymphocyte proliferation under heat stress.Methods Male BALB/C mice are divided randomly into a water supplement group,four groups〔0 5,1 5,2 5,3 5?g/(kg.bw)of L-Arg〕,each with 35?mice,normal temperature contrast group with 5 ones .After 14 days,all the mice are treated with heat stress[(41±0 5)℃,120?min]expect for normal temperature contrast group.After heat stress,round-the -clock every two hours,get blood from eye socket separately and tail for the mice of normal temperature contrast group at 3,7,11,14?days.To obverse index and determine the content of the NO in serum and the cell activity of the mice's lymphopoiesis.Results Under the normal temperature,the content of the NO in serum reaches maximum after three days of the arginine supplement,and reaches stabilization 7 days later,groups at any time is higher in the supplement than not supplement groups,and the group of 0 5?g/(kg.bw)is the most in all supplement groups.Under the heat stress,the content of the NO in serum decreases first and increases later in these groups of the 0 5,1 5?g/(kg.bw)and not supplement group.After 4-8?h,it reaches stabilization.However,in high content groups〔2 5 and 3 5?g/(kg.bw)〕,NO rises steadily after high stress,and during the 12?h,the content of NO is higher than the former ones.Under heat stress,concanavalin A(ConA)stimulation exponent in the supplement group is higher than compared groups.In all supplement groups,ConA stimulation exponent of the 1 5?g/(kg.bw)group reach the most.After heat stress,the cell activity of the little mouse's lymphopoiesis is restrained,and during 4 to 8?h,it reaches minimum.Conclusion The mouse requires more arginine in heat stress,but the changes of NO in serum concerns with the arginine's use.Lymphopoiesis depends on the adjustment of moderate NO in serum.More arginine supplenents product excess NO and restrain the multiplication of the cells.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2005年第3期304-306,共3页
Chinese Journal of Public Health
关键词
精氨酸
一氧化氮
细胞增殖
arginine
nitric oxide(NO)
lymphopoiesis
