摘要
目的探讨高强度聚焦超声(HIFU)三维周边式辐照离体灌注猪肝阻断其内部血供的可行性。方法对20块离体灌注猪肝行HIFU三维周边式辐照,辐照前后以肉眼和光镜观察ROI肝组织,并对标本行MR平扫及增强扫描。结果肉眼可见灌注猪肝辐照区均出现完整封闭的凝固性坏死带,内部区无明显变化;光镜下可见辐照区细胞核固缩、碎裂,内部区细胞肿胀、肝血窦扩张,辐照区与外周组织界限清晰,小叶间静脉内血栓形成。平扫MRI中,HIFU辐照前ROI表现为T1WI和T2WI等信号,辐照后辐照区T1WI信号降低、T2WI信号增高,辐照前后内部区T1WI和T2WI信号无明显变化;增强MRI中,辐照前ROI呈现强化、血流灌注丰富,辐照后内部区未见增强、血流灌注消失。结论 HIFU三维周边式辐照可有效阻断离体灌注猪肝ROI内部血供。
Objective To investigate the feasibility of HIFU 3D-peripheral scanning on blocking the local blood supply of isolated perfused pig liver tissue.Methods HIFU 3D-peripheral scanning was performed on ROI of 20 pieces of isolated perfused pig liver tissue.Visual and light microscopic observation,plain and contrast-enhanced MR scanning were performed before and after HIFU irradiation.Results Complete close zone of coagulative necrosis in irradiated area displayed in all tissues in visual observation,while the internal area showed no significant changes.Light microscopic observation found nucleus pyknotic and cataclastic in irradiated cells,while edema existed in internal cells,and hepatic sinusoid dilated,the boundary between irradiated and peripheral tissue was clear,with thrombosis formed in interlobular veins.In irradiation area,signals of ROI decreased on MR T1WI but increased on T2WI,while there was no significant changes in internal area after irradiation.Contrast-enhanced MRI showed that the blood perfusion in internal area disappeared.Conclusion HIFU 3D-peripheral scanning can effectively block the blood supply of the internal area of ROI in isolated perfused pig liver tissue.
出处
《中国介入影像与治疗学》
CSCD
2013年第2期65-68,共4页
Chinese Journal of Interventional Imaging and Therapy
基金
国家自然科学基金(81127901)
关键词
高强度聚焦超声消融术
周边式辐照
灌注
血供
体外
High-intensity focused ultrasound ablation
Peripheral scanning
Perfusion
Blood supply
In vitro
