摘要
为了解贵州省2011年诺如病毒的基因型,监测了2011年5月至12月于贵州省人民医院就诊的急性胃肠炎病例,收集粪便标本,采用荧光定量逆转录-聚合酶链反应(Real-time RT-PCR)初步鉴定,并对阳性株的VP1基因区克隆及测序。检测标本70份,GⅠ型诺如病毒阳性1株,阳性率1.43%,GⅡ型诺如病毒阳性34株,阳性率48.57%,获得7份GⅡ型诺如病毒VP1基因序列,3株为GⅡ.4亚型,为新型变异株(GⅡ4 2011),与GⅡ4 2006b变异株的亲缘关系最近,有1个氨基酸位发生了回复突变;4株为GⅡ.3亚型,分为2个基因簇,1簇与2008～2009年韩国株(HM635118)及上海株(GU991355)的亲缘关系最近,1簇与2010年日本株(AB629943)及2007年印度株(EU921389)等的亲缘关系最近,有4个氨基酸位点易发生回复突变。
To study the genotype of Norovirus associated with acute gastroenteritis in Guizhou Province 2011 , the patients＇ fecal specimens were collected from the Guizhou Province People＇s Hospital in the pe- riod of May to December 2011. Noroviruses in specimens were detected by a real-time reverse transcription polymerase chain reaction （Real-time RT-PCR）. VP1 genes of norovirus-positive strains were then cloned and sequenced . Out of 70 clinical samples, the positive rates for norovirus G I （1 strain） and G Ⅱ （34 strains） were 1. 43% and 48. 57%, respectively. The VP1 sequencing results of seven norovirus G II showed thatthree strains were genotype G H 4 and four strains were genotype G Ⅱ 3 Those genotype G Ⅱ. 4 strains were new variants （GII. 4 2011）,closest to G 11.4 2006b variant. One amino acid appeared back mutation. Those genotype G Ⅱ.3 strains were divided into 2 gene clusters. One cluster was closest to Korean strain （HM635118） and Shanghai strain（GU991355）. One cluster was closest to Japaness strain （AB629943） and 2007 Indian strain （EU921389）, Four amino acids appeared back mutations
出处
《病毒学报》
CAS
CSCD
北大核心
2013年第1期51-55,共5页
Chinese Journal of Virology
基金
国家科技重大专项"传染病监测技术平台"项目(2009ZX10602-12)
