摘要
目的:优选芦荟的保湿活性成分,并对其进行皮肤适应性评价。方法:采用皮肤水合状态和经皮水分散失测试的方法,优选芦荟中的保湿活性成分;采用红细胞溶血、Franz扩散池体外透皮吸收以及人体斑贴试验,分别从生物相容性、皮肤渗透性与皮肤安全性3个方面综合评定保湿活性成分的皮肤适应特性。结果:芦荟的多糖成分有显著提高皮肤水合状态(P<0.05),同时降低经皮失水(P<0.05)的优良保湿功效;芦荟多糖液(2,20 g.L-1)对红细胞的溶血率均低于0.3%,远低于血液相容性的常规值,与人体相容度高;5%芦荟多糖皮肤渗透速率达到0.251 mg.h.cm-2,24 h累计渗透量达(4.152±0.046)mg.cm-2,具备较强的皮肤渗透力;人体皮肤斑贴试验中全部评定为0级反应,对人体高度安全。结论:库拉索芦荟活性多糖对皮肤具有显著的保湿功效以及优良的皮肤适应性,可作为保湿类化妆品或外用皮肤干燥症制剂中的优良添加剂在皮肤科中广泛应用。
Objective： To optimize Aloe moisturizing active ingredients and to evaluate their skin adaptability. Method： Moisturizing active ingredients of Aloe were optimized with the skin hydrated state and transepidermal water loss test method. Skin adaptability of the moisturizing active ingredients was evaluated by erythrocyte hemolysis method, franz diffusion cell in vitro transdermal absorption method and the human skin patch test method, respectively from the biocompatibility, skin permeability and skin safety three aspects. Result： Aloe polysaccharide components showed excellent moisturizing efficacy, significantly improving skin hydration status （P 〈0.05）, at the same time, reducing the skin water loss （P 〈 0.05）. Different concentrations of aloe polysaccharide solution （2, 20 g L-1 ） to the erythrocyte hemolysis rate was lower than 0.3 % , far below the blood compatibility of the conventional value, with human body compatibility degree was high. Polysaccharide mass concentration was 5%, the skin penetration rate was 0. 251 mg h cm-2, and the quantity of the transdermal absorption reached （4. 152 ±0. 046） mgcm-2 in 24 hours; in the human skin patch test all evaluation showed zero level reaction, so it should be highly safe for human application. Conclusion： Aloe active polysaccharide has significant moisturizing efficacy and good skin adaptability. It can be widely used in dermatology, as a excellent moisturizing cosmetics additive.
出处
《中国实验方剂学杂志》
CAS
北大核心
2013年第3期252-256,共5页
Chinese Journal of Experimental Traditional Medical Formulae
基金
"十二五"国家科技支撑计划课题(2012BAD36B02)
关键词
芦荟
保湿
生物相容性
透皮吸收
安全性
Aloe
moisturize
bioeompatibility
transdermal absorption
safety
