摘要
目的:探讨舌癌组织中高迁移率族蛋白1(HMGB1)表达情况。方法:采用免疫组化SABC方法和高分辨率溶解法,检测43例舌癌组织、11例正常舌组织中HMGB1蛋白及mRNA的表达情况,分析其与肿瘤大小、分化程度、转移和复发的关系。结果:HMGB1蛋白在舌癌组织中表达强阳性率为72.1%,弱阳性率为27.9%,在正常舌组织中弱阳性表达(P<0.01),HMGB1mRNA在舌癌组织的定量明显高于正常组织(P<0.05);HMGB1表达强度与舌癌分化程度无关(P>0.05),与病灶大小、淋巴结转移、临床分期、复发相关(P<0.05)。结论:HMGB1在舌癌组织中呈强阳性表达,可作为舌癌复发、转移及预后的判定指标之一。
Objective： To observe the expression of high mobility group box 1 protein（ HMGB1 ） in human tongue squamouse cell carcinoma（TSCC）. Methods： Immunohistoehemical SABC method and high-resolution melting were used to detect the expression of HMGB1 in 43 cases of TSCC and 11 cases of normal tongue tissues. The relationship between HMCB1 expression and differentiation degree, the size, metastasis and recurrence of TSCC was analysed. Results： The strong positive expression rate of HMGB1 was 72.1% and weak positive expression rate was 27.9% in TSCC, and weak positive expression was observed in normal tongue tissues（ P 〈 0.01 ）. The HMGB1 mRNA expression was higher in TSCC than in the control tongue tissue（ P 〈 0.05） The expression of HMGB1 was correlated to tumor size, metastasis and recurrence of TSCC （P 〈 0.05 ） , but had no relationship with the differentiation degree（ P 〉 0.05 ）. Conclusion： The strong expression of HMGB1 TSCC might be as one of the parameters for the prognosis.
出处
《实用口腔医学杂志》
CAS
CSCD
北大核心
2013年第1期76-79,共4页
Journal of Practical Stomatology
基金
厦门市科技计划项目(编号:3502Z20084035)
关键词
舌癌
高迁移率族蛋白1
免疫组织化学
高分辨率溶解
Squamouse cell carcinoma of tongue
High mobility group box protein 1 （ HMGB1 ）
Immunohisto-chemistry
High- resolution melting
