摘要
蛋白质组学(Proteomics)是一门大规模、高通量、系统化的研究某一类型细胞、组织或体液中的所有蛋白质组成及其功能的新兴学科。虽然基因决定蛋白质的水平,但是基因表达的水平并不能代表细胞内活性蛋白的水平,蛋白质组学分析是对蛋白质翻译和修饰水平等研究的一种补充,是全面了解基因组表达的一种必不可少的手段。蛋白质组学相关技术的发展极大地推动了蛋白质组学的研究进展,使其在各研究领域得到了广泛的应用。对蛋白质组学相关技术及其在各领域的应用进行了综述,最后对蛋白质组学的发展趋势和应用前景作出展望。
Proteomics is an emerging discipline for studying proteins composition and function in a type of cell, tissue or body fluids in a large-scale, high-throughput and systematic level. While genes determine the level of protein, but the level of gene expression can not represent the intracellular reactive protein levels. Proteomic analysis is a complement to the study of translation and modification and also an indispensable tool for a comprehensive understanding of genome expression. The development of proteomic technologies has greatly promoted the progress of proteomic research, and it has been widely used in various research fields.This paper revieweded the proteomic technologies and the applications in various fields are also briefly reviewed. Finally, some future issues are presented.
出处
《生物技术通报》
CAS
CSCD
北大核心
2014年第1期32-38,共7页
Biotechnology Bulletin
基金
甘肃省科技计划基金资助项目(0708NKCA129)
兰州大学第二医院医学研究基金项目(YJ2010-08)
关键词
蛋白质组学
双向凝胶电泳
质谱
生物信息学
应用现状
Proteomics Two-dimensional gel electrophoresis Mass spectrometry Bio-informactics Application status
