摘要
目的研究强直性脊柱炎（As）患者外周血单个核细胞（PBMCs）中微小RNA（miR）．146a、肿瘤坏死因子受体相关因子6（TRAF6）和白细胞介素-1受体相关激酶1（IRAKl）的表达及其与病情活动度的相关性，探讨miR．146a、TRAF6、IRAKl在AS发病机制中的作用。方法采用实时荧光定量聚合酶反应（qRT-PCR）检测45例As患者miR-146a．TRAF6、IRAKl的相对表达量与22名健康对照组比较，并与BathAS疾病活动指数（BASDAI）、红细胞沉降率（ESR）、C反应蛋白（CRP）、免疫球蛋白（Ig）等进行相关分析。统计学处理采用t检验，非参数检验，单因素方差分析，Pearson、Spearman相关分析。结果①miR-146a在AS患者PBMCs中相对表达量显著高于健康对照组[1．46（0．39，4．79）与0．81（0．17，1．90），P〈0．05]、活动组显著高于稳定组[2．93（0．95，7．95）与0．54（0．28，1．69），P〈0．05]、用药组与未用药组差异无统计学意义[1．28（0．31，2．37）与2．22（0．49，7．71），P〉0．05]；②IRAKl表达水平明显高于健康对照组（1．4±0．7与1．1±0．4，P〈0．05），未用药组与用药组、活动组与稳定组表达量差异均无统计学意义（1．5＋0．9与1．4±0．5；1．6±0．7与1．3±0．7，P〉0．05）；③TRAF6表达量明显低于健康对照组（1．3±0．6与1．7±0．8，P〈0．05），TRAF6在未用药组、活动组均明显低于健康对照组（1．1＋0．7与1．7~0．8；1．1±0．5与1．7＋0．8，P〈0．05）。④AS患者miR．146a表达与BASDAI、晨僵时间呈正相关（r=0．557，P=-0．000；r=0．363，P=O．018），IRAKl与IgM呈负相关（r=-O．313，P=0．046）。结论①miR-146a可能参与As疾病活动；②IRAKl、TRAF6表达异常可能在AS的发病机制中起作用。
Objective To investigate the expression of micro RNA-146a （miR-146a）, TNF receptor- associated factor 6 （TRAF6） gene and IL-1 receptor-associated kinase 1 （IRAK1） gene in the peripheral mononuclear cells （PBMCs） of patients with ankylosing spondylitis （AS） and their relationship with the disease activity. The role of miR-146a, TRAF6, IRAK1 in the pathogenesis of AS was explored. Methods Expression of miR-146a, TRAF-6 and IRAK-1 in peripheral blood mononuclear cells was studied using real- time polymerase chain reaction （qRT-PCR） in 45 AS patients and 22 healthy controls. The indicators of disease activity adopted in this study were Bath ankylosing spondylitis disease activity index （BASDAI）, erythrocyte sedimentation rate （ESR）, C-reactive protein （CRP） level, and immunoglobulin （Ig）. The relationship was analyzed in AS patients between the relative expression levels miR-146a, TRAF6, IRAK1 and BASDAI, ESR, CRP, Ig concentration. Non-parametric＂ test, t test, One-way ANOVA, Pearson＇s and Spearman＇s correlation analysis were used for statistical analysis. Results （1）The relative expression level ofmiR-146a which was observed in PBMCs of AS patients was significantly higher than that in normal control group [ 1.46 （0.39, 4.79 ） and 0.81 （ 0.17, 1.90 ）, P〈0.05 ]. The expression of miR- 146a was significantly higher in active AS patients group than that in inactive patients [2.93（0.95, 7.95） and 0.54（0.28, 1.69）, P〈0.05], there was no difference between the treatment group and without treatment group [ 1.28（0.31, 2.37） and 2.22 （0.49, 7.71 ）, P〉0.05 ]. （2） There was significant difference in the relative expression level of IRAK-1 between AS patients and the normal control group. IRAK1 was significantly higher in AS patients than that in normal control group （1.4＋0.7, 1.1-＋0.4, P〈0.05）. However, there was not difference between active AS patients group and inactive patients group as well as treated group and untreated group �
出处
《中华风湿病学杂志》
CAS
CSCD
北大核心
2013年第2期119-123,共5页
Chinese Journal of Rheumatology
