摘要
目的研究NG2细胞在大鼠脊髓损伤白质内源性增殖及形态特征。方法成年SD雄性大鼠42只,随机平均分为模型组和假手术组。按课题组自行设计的方法制作脊髓压迫模型,假手术组仅暴露脊髓。分别于术后1、3、7d运用免疫组化检测脊髓内NG2细胞的表达。采用Image Pro Plus6.0软件对NG2阳性细胞计数并测量其胞体面积和突起长度。结果伤后1d,NG2+细胞增多(30.17±11.08)/视野,至3d达到高峰(90.75±9.40)/视野,7d后下降(78.38±8.91)/视野,但仍多于假手术组(19.92±6.68)/视野(P<0.05)。在假手术组,NG2+细胞平均胞体面积为(205.67±10.80)μm2、平均突起长度为(22.92±1.24)μm,伤后1d,NG2+细胞胞体变小(128.25±32.06)μm2、突起变短(10.98±4.25)μm,3d后胞体变大(225.26±16.64)μm2、突起增长(18.63±2.26)μm(P<0.05),至7d变化不明显(P>0.05)。在脊髓压迫损伤后,可见许多胞体较小呈圆形、突起少或无的NG2+细胞集落。结论在脊髓压迫损伤一周内,NG2细胞增殖活跃,胞体渐大,突起变长,但仍短于正常。
Objectivo To investigate endogenous proliferation and morphological characteristics of NG2-cells in the white matter of compressed spinal cord injury （ACSCI） of rats. Mothods 42 adult SD rats were randomly divided into 2 groups： model group （n=21）, which underwent spinal cord compression by a self-made device, and sham group （n=21）, which underwent only laminectomy. Expression of NG2-positive cells was detected by IHC after 1, 3 and 7 days. The average number, soma area, process length of NG2＋-cells were measured by Image Pro Plus6.0. Results Compared with control group （19.92±6.68 per field）, the number of NG2＋-cells increased at ld （30.17±11.08 per field）, peaked at 3d （90.75 ±9.40 per field）, and decreased at 7d （78.38±8.91 per field） （P〈0.05）. NG2＋-cells＇ soma became smaller （128.25±32.06 μm2） and processes became shorter （10.98±4.25 μm） at ld, then NG2＋-cells＇ soma became larger （225.26±16.64 μm2） and processes became longer （18.63±2.26 μm） at 3d （P〈0.05）. However, the soma area and process length of NG2＋-cells at 7d kept unchanged compared with that at 3d （P〉0.05）. There were many clusters ofNG2＋ cells that had smaller and round soma, fewer or no processes after CSCI. Couclusion NG2＋-cells could proliferate actively with the soma growing larger and the process growing longer but still shorter than normal in CSCI after a week.
出处
《中国临床解剖学杂志》
CSCD
北大核心
2013年第1期81-84,共4页
Chinese Journal of Clinical Anatomy
基金
国家自然科学基金(30270437)
关键词
脊髓压迫伤
NG2细胞
内源性增殖
细胞形态
Compressed spinal cord injury
NG2 cells
Endogenous proliferation
Cellular morphology
