摘要
目的建立反相高效液相色谱法，测定丁香挥发油中丁香酚的含量。方法采用Lichrospher C18色谱柱（150mm×4．6mm，5μm），流动相为甲醇-水（64：36），柱温25℃，流速为1．0ml／min，检测波长280nm，以外标法峰面积定量。结果丁香酚与挥发油中其他成分的分离效果良好，在0．11～110．00mg／L浓度范围内呈良好的线性关系，回归方程为：Y=17．74C＋1．518（r=0．9999，n=5）；平均回收率为100．06％，相对标准偏差为0．16％。结论本方法简便、准确，可快速测定丁香挥发油中丁香酚的含量，可为质量控制提供依据。
Objective To establish a reversed-phase high performance liquid chromatography （RP-HPLC） for simultaneous determination of eugenol in the essential oil from Clove. Method The mobile phase was carbinolwater（64： 36） and the flow rate was about 1.0 ml/min. Lichrospher C18 （150 mm ×4.6 mm, 5 μm） column was used and the detective wavelength was 280 nm. Results The calibration curves were linear in the concentration range of 0.11 -110.00 mg/L for eugenol. The regression equation was Y = 17.74 C ＋ 1. 518 （ r = 0. 9999, n = 5 ） , the average recovery was 100.06% and the relative standard deviations was 0.16%. Conclusion Our established method is simple, quick and accurate for the simultaneous separation and determination of eugenol in the essential oil from Clove.
出处
《中国医药》
2013年第2期248-249,共2页
China Medicine
关键词
反相高效液相色谱法
丁香酚
丁香挥发油
Reversed-phase high performance liquid chromatography
Eugenol
Essential oil from Clove
