摘要
该文采用PCR方法扩增获得了纳豆激酶基因(pro-NK),通过酶切与酶连反应使其与原核表达载体Pet32a相连,构建重组质粒Pet32a-pro-NK,先转入到大肠杆菌DH5α中进行筛选与验证,再将阳性克隆子转化到宿主菌BL21(DE3)中,在IPTG诱导下进行纳豆激酶蛋白的表达研究。SDS-PAGE电泳分析结果表明,纳豆激酶蛋白在BL21(DE3)中获得高效表达。
The nattokinase gene pro-NK was cloned by PCR technique.The expression plasmid of Pet32a-pro-NK was constructed based on enzyme digestion and enzyme-linked reaction.The confirmed positive clones were further transformed into Escherichia coli strain BL21（DE3） to study the expression of nattokinase with IPTG induction.The result of SDS-PAGE electrophoretic analysis manifested that nattokinase protein could express efficiently in BL21（DE3）.
出处
《江西农业学报》
CAS
2012年第12期144-146,共3页
Acta Agriculturae Jiangxi
基金
江西省科技计划项目"纳豆激酶蛋白在酿酒酵母中的表达及应用"(20112BBF60009)
关键词
纳豆激酶基因
克隆
表达
大肠杆菌
Nattokinase gene
Cloning
Expression
Escherichia coli
