摘要
考察了诃子提取物及其色谱纯化物(a、b、c)清除自由基的活性能力,进行了1,1-二苯基-2-苦肼基(DPPH.)清除试验。结果表明,甲醇提取物、乙酸乙酯提取物及其色谱纯化物均具有清除自由基活性的能力,甲醇粗提物活性略高于等体积1 mg/mL TBHQ,而其色谱纯组分c的活性最高,约为1 mg/mL TBHQ的1.2倍,且略高于茶多酚;乙酸乙酯组分色谱纯组分a,b抑制率分别为其粗提取物的1.6和1.1倍。色谱纯成分经显色反应、UV和H1NMR检测后被确认为诃黎勒酸衍生物或诃子单宁水解碎片,此为诃子提取物作为烟用自由基清除剂提供了有效的理论依据。
The free-radical-scavenging activity of different groups from terminalia chebula ritz were studied. The results showed that all extracts of terminalia chebula ritz and three chromatographically purified components had strong DPPH. scavenging effects. The methanol extract in 1 mg/mL showed that it had better free-radical-scavenging activity than that of TBHQ and its chromatographically purified component C showed the strongest activity among all samples. Its activity was slightly stronger than that of tea polyphenol, as 1.2 folds of TBHQ＇s. The activities of two chromatographically purified components from ethyl acetate extract were 1.6 and 1.1 folds as the original, respectively. By color test, UV and H1NMR all of the purified components were confirmed to be the derivatives of myrobalani-tannin or their hydrolyzed segments. The extracts of terminalia chebula ritz as free-radical-scavenging of tobacco provided an effective theoretical basis.
出处
《中国烟草科学》
CSCD
2012年第6期97-101,共5页
Chinese Tobacco Science
