摘要
目的:研究楤白皮总皂苷的体内外抗肿瘤活性。方法:采用MTT体外测定楤白皮总皂苷对小鼠B16黑色素瘤、小鼠H22肝癌细胞和小鼠FBL3红白血病细胞的增殖抑制作用;采用小鼠移植性肿瘤S180和H22观察楤白皮总皂苷的体内抑瘤活性。结果:楤白皮总皂苷体外对B16细胞、FBL3细胞的IC50分别为0.077 4 g.L-1和0.199 g.L-1;在0.02～3.2 g.L-1范围内,对H22细胞体外抑制率均为80%以上。在100、200、300 mg.kg-1剂量下,楤白皮总皂苷对S180小鼠肉瘤的抑瘤分别为16.9%、37.1%和27.0%,对H22小鼠肝癌的抑瘤率分别为23.7%、10%和13.4%。结论:楤白皮总皂苷在体内、外均具有一定的抗肿瘤活性。
OBJECTIVE To investigate the anti-tumor effect of total saponins of Aralia white （TSAW） in vitro and in vivo. METHODS The cytotoxicity of TSAW to mouse B16 melanoma ceils, H22 hepatoma cells and FBL3 erythroleukemia cells were tested by cell inhibitory assay. The inhibitory effect of TSAW on S180 and H22 tumor growth were observed in mice. RESULTS The proliferations of B16 cells, FBL3 ceils and mice H22 cells were inhibited by TSAW in vitro. IC50 of I316 and FBL3 were 0. 077 4 g.L-1 and 0. 199 g.L-1 respectively. The inhibitory rate of TSAW on H22 cells proliferation was more than 80% in the concentration range 0. 02 - 3. 2 g.L-1 in vitro. The inhibitory rates of TSAW on S180 tumor growth were 16. 9%, 37. 1% and 27. 0%, at 100, 200 and 300 mg.kg oral dose respectively in vivo, and on H22 tumor weight were 23. 7%, 10% and 13. 4% at the same dosage series. CONCLUSION TSAW showed antbtumor activities in vitro in the concentration range 0. 02 - 3.2 g. L -1 and in vivo with the oral dosage of 200 - 300 mg.kg -1 in mice.
出处
《中国医院药学杂志》
CAS
CSCD
北大核心
2013年第2期93-97,共5页
Chinese Journal of Hospital Pharmacy
基金
国家自然科学基金(编号:81071765)
教育部留学归国人员科研启动基金(41批
11回国基金02)
