摘要
以山杏为试材,研究了外植体的消毒、基本培养基筛选、外植体的种类及不同浓度配比的激素对外植体各阶段生长的影响。结果表明:采用1.0g/L的HgCl2消毒3min灭菌效果最好;以改良MS[C(NH4+)∶C(NO3-)=0.16]为基本培养基,组培苗分化和生长效果较好;最适外植体为茎尖;初代培养最适的激素配比为1.0mg/L的6-BA和0.05mg/L的NAA;继代培养最适的激素配比为0.5mg/L的6-BA和0.1mg/L的NAA。
The Prunus sibirica was used as material, the kind of basic medium, the kind of explants and the different proportions of hormones impact the growth condition of explants in the various stages of tissue culture were studied. The results showed that the best sterilization effect was used HgO12 1 g/L as disinfectants to disinfect 3 rain;the division and growth condition of explants was better in improved MS[C(NH4+) : C(NOa-)=0. 16]medium; the most suitable explants was shoot tips;the most suitable proportions of hormones was 6-BA 1.0 mg/L and NAA 0. 05 rng/L in primary culture;the most suitable proportions of hormones was 6-BA 0. 5 mg/L and NAA 0. 1 mg/L in subculture.
出处
《北方园艺》
CAS
北大核心
2013年第3期115-118,共4页
Northern Horticulture
基金
国家林业公益性行业科研专项资助项目(201004034)
中央财政林业科技推广示范资金资助项目([2010]02)
关键词
山杏
组培
繁殖技术
Prunus sibirica
tissue culture
proportion technology