摘要
以响叶杨×84K杨的茎段和叶片为外植体,应用正交实验方法对响叶杨×84K杨的再生条件进行研究。结果表明:茎段和叶片不定芽诱导的最佳培养基分别为MS+TDZ 0.0015mg/L+NAA0.05 mg/L,茎段不定芽再生率可达91%,MS+TDZ 0.001 mg/L+NAA0.01mg/L,叶片不定芽再生率可达70%;诱导不定芽生根的最佳组合为1/2MS+NAA0.01 mg/L+IBA0.1 mg/L,生根率可达100%。在碳源对生根率影响的研究中,蔗糖的影响最大,生根率可达88.9%。
Through the application of orthogonal designing, stems and leaves of Populusadenopoda × the poplar 84K were used as explants to study the optimal differentiation medium and proportion of hormone. The results showed that the optimal differentiation medium and proportion of hormone of stems and leaves were respectively MS＋TDZ 0. 0015 mg/L＋ NAA 0. 05 mg/L and MS＋TDZ 0. 001 mg/L＋NAA 0. 01 mg/L, and 1/2MS＋NAA 0. 01 mg/L＋IBA 0. 1 mg/L for adventitious root. Using the optimal regenertion system, regeneration rate of stems was 91%, regeneration rate of leaves was 70%, rooting frequency was 100%. In the study of rooting rate on carbon, the impact of sucrose was largest, on which the rate of rooting was 88. 9%. The results achieved laid a sound foundation for regeneration of Populusadenopoda × poplar 84k on an industrial basis and gene transformation by means of leaf disc method.
出处
《北方园艺》
CAS
北大核心
2010年第9期152-156,共5页
Northern Horticulture
基金
国家"十一五"科技支撑计划资助项目(2006BAD01A1502-2)
陕西省13115市大科技专项资助项目(2009-13)
关键词
响叶杨×84K杨
植株再生
组织培养
Populusadenopoda ×the poplar 84 K
plantlet regeneration
tissue culture
