摘要
目的应用微电极阵列芯片(microelectrode arrays chip,MEA)技术评价48 h房颤(atrial fibrillation,AF)犬左、右心耳(LAA、RAA)的电生理特性。方法随意来源犬12只,以600次/分起搏右心房建立AF模型,分为48 h AF组(n=6)和对照组(n=6)。造模成功后迅速开胸剪取LAA、RAA,置于盛有台式液的MEA中,分别记录AF组及对照组LAA、RAA场电位(field action potential,FAP)形态、振幅、放电频率及激动传导情况。结果 AF组LAA、RAA组织FAP节律绝对不齐,LAA(185.22±25.62)次/分,较对照组(156.44±8.88)次/分增加15.67%(P<0.01),RAA(102.39±16)次/分,较对照组(156.44±8.88)次/分减慢34.62%(P<0.01)。48 h AF组LAA组织电压(458.33±26.73)μV较对照组(740.55±18.93)μV降低38.11%(P<0.01),RAA(504.83±39.93)μV较对照组(840.56±18.93)μV明显降低(P<0.01),48 h房颤组LAA组织FAP时程(45.28±8.59)ms较对照组(70.77±6.98)ms缩短15 ms(P<0.01)。RAA(61.78±7.1)ms较对照组(75.83±7.63)ms缩短14 ms(P<0.01)。48 h AF组LAA、RAA FAP传导异质性增加。结论应用MEA技术可反映心肌组织片场电位电生理特性,48 h AF后LAA放电频率增加,频率绝对不齐,LAA、RAA电压降低,场电位时程延长。
Objective To explore the electrophysiological characteristics of right atrial appendage （RAA） and left atrial appendage （LAA） in 48-h atrial fibrillation （AF） canine detected by microeleetrode array （MEA） chip tech- nique. Methods Twelve adult healthy mongrel canine were randomly divided into two groups：48-h pacing group and con- trol group. The 48-h AF model was established by rapid pacing the right atrium. The RAA and LAA were quickly removed and immersed into Tyrode＇ s solution in MEA chip dish. The morphology, duration, voltage, discharge frequency and con- duction of field action potential were recorded. Results In the AF group, the rhythms of LAA and RAA tissue field poten- tial were irregular. The frequency of LAA （ 185.22 ± 25.62 beats/min） was increased by 15.67% （ 156. 44 ± 8.88 beats/ min） （ P 〈 0. 01 ）, and the frequency of RAA （ 102.39±16 beats/min） was decreased by 34. 62% than that in the control group （156. 44 ± 8.88 beats/min） （P 〈 0. O1 ）. The voltages of LAA （458.33 ± 26.73 ） μV and RAA （504. 83 ±39.93 ）μV were decreased than those of the control group （740. 55 ± 18.93）μV and （840. 56 ±18.93 ）μV, respectively, （P 〈 0. 01 ）. The durations of field potential of LAA （45.28± 8.59） ms and RAA （61.78 ± 7.1 ） ms were significantly shortened than those of the control group （70. 77 ±6. 98）ms （P 〈 0. 01 ）. The electric impulse presented anisotropic alteration. Conclusions MEA is a sensitive, long-lasting and stable technique, and local tissue action potential can be used to assess the electrophysiological characteristics of field potential in animal heart slices using multiple-channel recording mapping sys- tem. The discharge frequency of left atrial appendage is increased and frequency irregularity occurs after 48 h atrial fibrilla- tion, the voltage of field potential is decreased in the atrial appendage, and the field potential duration is prolonged.
出处
《中国实验动物学报》
CAS
CSCD
2012年第6期38-42,I0005,共6页
Acta Laboratorium Animalis Scientia Sinica
基金
国家国际科技合作专项项目(2011DFA32860)
国家自然科学资助基金项目(30960132)
关键词
心房颤动
微电极阵列
场电位
电生理学
左心耳
右心耳
随意来源犬
Atrial fibrillation
Microelectrode arrays chip
Field action potential
Electrophysiology
Left atrial appendage
Right atrial appendage
Canine, Random source dogs
