摘要
目的比较RT-PCR和FQ-PCR法在手足口病(HFMD)病原学检测中的差异,并探讨亳州地区EV71和CoxA16的流行特征。方法采集亳州地区2011年193例疑似HFMD患者不同种类的标本共252份。对其中的124份咽拭子样品,同时采用RT-PCR和FQ-PCR法进行EV71和Cox A16检测,并比较两种方法检测结果的差异。运用FQ-PCR法对所有标本进行检测,比较不同类型标本间结果的差异,并分析不同感染类型的阳性率。用FQ-PCR法检测咽拭子标本188份,分析不同类型HFMD感染者的季度分布。结果用FQ-PCR及RT-PCR法检测124份疑似HFMD患者的咽拭子标本,EV71的阳性率分别为68.55%(85/124)和63.71%(79/124),Cox A16的阳性率分别为46.77%(58/124)和41.94%(52/124)。两种方法检测结果间的差异均有统计学意义(χ2分别为95.385、101.908,P均<0.01)。用FQ-PCR法共检测252份标本,其中咽拭子、粪便、疱疹液和脑脊液的阳性率分别为75.00%(106/188)、81.13%(43/53)、6/7和1/4,不同类型标本之间阳性率的差异无统计学意义(χ2=6.888,P>0.05)。EV71单独感染、Cox A16单独感染及联合感染率分别为42.06%、9.92%和23.81%,其差异有统计学意义(χ2=63.369,P<0.05)。EV71单独感染季度间差异无统计学意义(χ2=6.561,P>0.05),而Cox A16单独感染及联合感染的季度间差异均有统计学意义(Cox A16单独感染:χ2=8.061,P<0.05;联合感染:χ2=24.898,P<0.01)。结论 FQ-PCR比RT-PCR法更适用于HFMD病原学检测,并且不受标本种类限制。EV71单独感染率最高,常年流行;联合感染次之,以夏秋季为主;Cox A16单独感染较少见,以秋冬季较多。
Objective To compare the differences between RT-PCR and FQ-PCR in etiological test of HFMD, and explore the epidemic characteristics of EVTI and Cox A16 in Bozhou. Methods 252 specimens of 4 kinds were collected from 193 clinically supposed HFMD patients in Bozhou area throughout 2011. A comparison was carried out between RTPCR and FQ-PCR, which were used for testing EV71 and Cox A16 in 124 throat-swab-specimens. The differences among the 4 kinds of samples were also estimated and the positive rates of the 3 infection types were calculated by testing all the specimens using FQ-PCR. 188 swab samples were tested with FQ-PCR kits as well, which was followed by the analysis on quarter distribution of HFMD types. Results FQ-PCR and RT-PCR were used to test 124 throat swabs ,which had been collected from the clinically supposed HFMD patients. The positive rates were 68.55 ~ （85/124） and 63.71% （79/124） for EV71,46. 77% （58/124） and 41.94% （52/124） for Cox A16 respectively . There was statistically significant difference between the 2 methods （Z2 were 95. 385 and 101. 908 respectively, P〈0. 01）. All the 252 specimens were tested with FQPCR kits. The positive rates of throat swabs,feces,herpes juices and cerebrospinal fluid were 75.00% （106/188）, 81.13% （43/53）,6/7 and 1/4 respectively, there were no statistically significant differences among the 4 types of specimens （χ^2= 6. 888,P〉0.05）. The infection rates of EV71 and Cox A16 were 42.06% and 9.92% respectively, the corresponding figure of joint infection was 23. 81%, there were statistically significant differences among the 3 groups （χ^2= 63. 369, P 〈0. 05）. There were no statistically significant differences for single EV71 infection among 4 quarters of 2011（χ^2= 6. 561,P 〉0.05） ,but the corresponding differences for Cox A16 infection and joint infection were significant （Cox A16：χ^2= 8. 061, P〈0. 05;joint infection：χ^2=24. 898,P〈0. 01）. Conclusions FQ-PCR is more suitable for HFMD testing than RT-PCR,
出处
《临床输血与检验》
CAS
2013年第1期11-15,共5页
Journal of Clinical Transfusion and Laboratory Medicine
基金
安徽省教育厅自然科学课题(No.KJ2011A181)
安徽省卫生厅临床医学课题(No.2010C052)资助
