摘要
目的了解2007-2009年青岛地区引起手足口病的人肠道病毒71(EV71)型VPI基因编码区(CDS)分子流行病学特征。方法采用荧光定量PCR对咽拭子标本进行总肠道病毒(EV)、柯萨奇病毒A16(CA16)和EV71检测。选取部分EV71检测阳性的标本进行VP1基因CDS序列的扩增及测序,对所获得的序列进行进化树分析。结果青岛市2007-2009年的51株EV71病毒核苷酸和氨基酸的差异性分别为5.3%和1.7%,所有病毒均属于C4亚型中的C4a簇。2008和2009年的基凶进化树均有多个分支产生,但每年都有一个主要分支(即优势株)。2008年的优势株与2007年山东省临沂市优势株高度同源,2008年的次优势株和2009年的优势株同与2008年安徽省阜阳市优势株高度同源。2007年的2株EV71与2007年山东省临沂市流行株高度同源。结论青岛市2008和2009年的EV71均有多个传播链存在,2008年的主传播链至2009年变为次传播链,但2008年的次传播链至2009年发展为主传播链。
Objective To study the molecular epidemiology of human enterovirus 71 (EV71) isolated from patients with hand-foot-mouth disease (HFMD) in Qingdao region between 2007 and 2009. Methods Throat swabs of HFMD were detected for total enterovirus (EV), EV71 and CA16 by fluorescence reverse transcription-polymerase chain reaction (RT-PCR). The VP 1 region was amplified from positive EV71 specimens and 51 nucleotide sequences of VP1 gene were successfully sequenced. EV71 genotypes were characterized by phylogenetic analysis. Results 51 Qingdao EV71 strains identified between 2007 and 2009 belonged to C4a cluster of subgenotype C4 and 5.3% nucleotide divergence and 1.7% amino acid divergence were found among them. The strains in 2008 and 2009 were respectively divided into several lineages on phylogenetic trees but there was a main lineage (namely prominent strain) every year. The Qingdao prominent strains identified in 2008 had high identities with the Linyi prominent strains in 2007. The Qingdao prominent strains in 2009 and the secondary prominent strains in 2008 had high identities with the Fuyang prominent strains in 2008. Two Qingdao strains in 2007 had high identities with the Linyi popular strains in 2007. Conclusion Data showed that several EV71 transmission chains were co-circulating in Qingdao between 2008 and 2009. The main transmission chain in 2008 became the minor one but the minor transmission chain in 2008 was transferred into the main transmission chain in 2009.
出处
《中华流行病学杂志》
CAS
CSCD
北大核心
2011年第4期382-384,共3页
Chinese Journal of Epidemiology
基金
本研究得到青岛市科技局项目(08-2-1-1-nsh)的支持