摘要
目的探讨鼻炎冲剂治疗变应性鼻炎的免疫调节机制。方法选择30例常年性变应性鼻炎患者,予鼻炎冲剂连续口服2月,进行疗效评估;采用流式细胞仪测定20例正常对照组及30例患者组治疗前后外周血单个核细胞的CD4+CD25+Foxp3+T细胞占CD4+CD25+T细胞比例;同时采用ELISA法测定血清IL-10、IFN-γ的含量。结果治疗组有效率为86.7%;外周血单个核细胞中CD4+CD25+Foxp3+T细胞占CD4+CD25+T细胞比例:治疗组治疗前16.83±2.94%,治疗后19.38±3.71%;对照组20.75±4.64%。血清IL-10含量(pg/ml):治疗组治疗前6.87±1.08,治疗后11.97±1.62;对照组13.18±1.56。血清IFN-γ含量(pg/ml):治疗组治疗前14.77±2.13,治疗后14.56±2.29;对照组15.65±1.93。结论本研究提示鼻炎冲剂可通过提高外周血单个核细胞中CD4+CD25+Foxp3+T细胞数量及血清IL-10含量有效治疗变应性鼻炎。
Objective To study the immunomodulatory mechanism on Granule-Rhinitis in the Treatment of allergic rhinitis（AR）. Methods In our study, 30 patients with AR received Granule-Rhinitis treatment for 2 months. Nasal symptoms scores were assessed to evaluate the clinical eficacy. Peripheral blood was obtained from 30 patients with AR and 20 normal controls. The frequencies of CD4＋CD25＋Foxp3＋T cells in CD4＋CD25＋T cells found in PBMC were evaluated by using flow cytometry. The concentration odd IL-10, IFN-yin the sera were measured by double antibody sandwich enzyme-linked immunosorbent assay. Results The effective rate in treatment group was 86.7%. The frequencies of CD4＋CD25＋Foxp3＋T cells in CD4＋CD25＋T cells found in PBMC before and after treatment were 16.83±2.94% and 16.83±2.94% respectively, while the frequency in the control was 20.75±4.64%. The concentration odd IL-10 in the sera before and after treatment were 6.87±1.08 （pg/ml） and 11.97±1.62 （pg/ ml） respectively, while the frequency in the control was 13.18±1.56 （pg/ml）. The concentration odd IFN-y in the sera before and after treatment were 14.77±2.13 （pg/ml） and 14.56±2.29 （pg/ml）respectively. while the frequency in the control was 15.65±1.93 （pg/ml）. Conclusions This study originally provided the evidence that Granule- Rhinitis could treat AR efficiently through improving the number of CD4＋CD25＋Foxp3＋T ceils in PBMC and the concentration of odd IL-10 in the sera.
出处
《中国中西医结合耳鼻咽喉科杂志》
2012年第6期408-412,共5页
Chinese Journal of Otorhinolaryngology in Integrative Medicine
