摘要
目的 论证甾体激素与低剂量棉酚组合用药的雄性抗生育作用的可行性及其作用机制。方法 用低剂量棉酚 12 mg/ (kg· d) ,甲基睾丸素 2 0 mg/ (kg· d)、炔雌醇 10 0 μg/ (kg· d)喂服 Wistar大鼠 6周 ,部分取材 ,开始合笼 ;继续用棉酚 12 m g/ (kg· d)维持 12周 ,部分取材 ;余下大鼠停药 ,观察恢复情况。取材做大鼠睾丸精子计数、精子活动度 ,睾丸切片的形态学观察等。结果 用药 6周大鼠的生精过程受到不完全抑制 ,附睾精子全部失去活力。用药 6 + 12周大鼠的曲细精管 - 期数量比例为 (9.3± 3.4) % ,比对照组 (2 6 .8± 1.7) %显著下降 ; - 期数量比例为 (36 .7± 5 .0 ) % ,比对照组 (2 4.5± 2 .7) %显著上升 ; - 期数量比例为 (3.6± 0 .6 ) % ,比对照组 (6 .0±0 .4) %显著下降。附睾精子全部失去活力。结论 甾体激素与低剂量棉酚从不同的作用位点共同作用于生精过程 ,在短时间内完全可以达到抗生育效果 ;低剂量棉酚长时期作用 ,不仅在附睾行使其抗生育功能 ,还影响生精过程产生精子的质量。此雄性抗生育作用具有可逆性。
To study the feasibility and the morphological mechanism of combined use of steroid hormone (methyltestosterone and ethinyl estradiol) and low dose gossypol for antifertility in adult Wistar rats. Methods We used methyltestosterone 20 mg/(kg·d),ethinyl estradiol 100 μg/(kg·d), and gossypol 12 mg/(kg·d) to feed male rats for 6 weeks via gastric intubation to induce infertility, then used gossypol 12 mg/(kg·d) alone for another 12 weeks to maintain infertility. Testicular sperm counts, cauda epididymal sperm motility, and sperm silver stainning were used to determine infertility and morphology of sperm at the end of the two phases. Testicular morphology of the rats was observed from HE and PAS staining of paraffin sections. F1 generation of the rats would be examined in a series of behavioral tests after their 6-week recovery periods. Results There was no substantial change in testicular sperm counts of drugs-treated rats both for 6 weeks and 6 plus 12 weeks compared with that of control. But cauda epididymal sperm motility of drugs-treated rats went down to zero at the end of two phrases. The result of sperm silver staining showed that epididymal sperm were all deleterious of either segregation of sperm nucha from sperm head, or separation in annular granule of sperm tail. The observation of testicular morphology suggested that spermatogenesis is only partially affected. The ratios of stagesⅡ-Ⅲ(9.3±3.4)% and stages Ⅸ-Ⅹ(3.6±0.6)% were remarkably droped, but that of stages Ⅳ-Ⅵ(36.7±5.0)% obviously ascented comparing with (26.8±1.7)%, (6.0±0.4)%, (24.5±2.7)% of control at the end of 6 plus 12 weeks. Moreover, no adverse effect was found in viscera tissue sections. Conclusions Steroid hormone made the procedure of spermatogenesis slower and low dose gossypol caused all sperm lose their activity in epididymis. Both affect the process of spermatogenesis from different endpoints and successfully induce infertility in short term. Low dose gossypol not only executs its antifertility function
出处
《中国医学科学院学报》
CSCD
北大核心
2000年第3期214-219,共6页
Acta Academiae Medicinae Sinicae
基金
国家自然科学基金! (396 70 76 3)
卫生部科学研究基金! (96 -1-0 74)资助
关键词
甾体激素
低剂量棉酚
避孕药
男性
steriod hormone
gossypol
antispermatogenic effect
