摘要
针对国内市场上流通的金鱼,开展CyHV-2的流行病学调查,并比较CyHV-2不同分离株的分子特征。结果显示:无明显临床症状的金鱼带毒率较高(13.89%),表明我国已有CyHV-2的分布。分子生物学研究显示,我国金鱼和异育银鲫来源的CyHV-2编码的解旋酶基因部分序列一致,表明异育银鲫来源的CyHV-2和金鱼来源的CyHV-2极可能为同一种病毒。同日本分离株(H.Fukuda)比较,我国金鱼来源的CyHV-2编码的DNA聚合酶基因片段序列同该毒株完全一致,但两者编码的衣壳体间三联蛋白和解旋酶各有1～2个氨基酸的差异,表明我国分布的CyHV-2同H.Fukuda毒株高度相似,但存在一定的差异。
Originally described from Japan, Cyprinid herpesvirus 2 （CyHV-2） was present in at least five other countries and probably has a global distribution in goldfish （Carassius auratus）. Up to now,CyHV-2 has drawn little attention and is rarely reported in China. Recently, silver prussian carp （Carassius auratus gibelio） farmed in Yancheng,Jiangsu Province,was reported by news to be infected by CyHV-2 and caused heavy economic losses. But little was known about the ways of virus transmis- sion, potential reservoirs and sources of infection for this newly emerged virus. Goldfish can be a source of infection by carrying CyHV-2 but showing no clinical symptom. To understand the epidemiology of CyHV-2 in China, healthy goldfish from markets in different districts were tested for CyHV-2 by degen- erate polymerase chain reaction of the viral polymerase gene followed by sequencing. Results demonstra- ted that CyHV-2 has a wide geographic distribution in China. Molecular study indicated that the se- quences of DNA polymerase encoded by CyHV-2 isolated from goldfish in China were identical with the published sequence for CyHV-2. The helicase sequences of CyHV-2 from goldfish were also partially i- dentical with those from silver prussian carp cultured in China, suggesting that the virus carried by gold- fish and silver prussian carp were the same. Between the isolates from goldfish and H. Fukuda from Ja- pan, there was only one or two residues difference in the amino acid sequence of helicase or intercapsom- eric triplex protein, suggesting that the two isolates of CyHV-2 were highly similar,but not the same.
出处
《华中农业大学学报》
CAS
CSCD
北大核心
2013年第1期92-96,共5页
Journal of Huazhong Agricultural University
基金
教育部博士点新教师基金(20100146120013)
中央高校基本科研业务费专项资金(52902-0900206094
2012ZYTS028)
湖北省杰出青年人才基金(2011CDA091)
关键词
鲤疱疹病毒
锦鲤疱疹病毒
流行病学
金鱼
Cyprinid herpesvirus 2
Koi herpesvirus
epidemiology
goldfish
