摘要
于江苏大丰金鹿合作社采集三种状态的异育银鲫Carassius auratus gibelio样本,试验1组(H)来自未发生过鲤疱疹病毒(Cy HV-2)病的2个池塘(面积200×667m2),即为无Cy HV-2病症状的病毒携带者;试验2组(IH)和试验3组(I)为正在发病的2个池塘(面积200×667m2),IH组有轻微的Cy HV-2病症状,而I组为典型的Cy HV-2病症状,测定其血成分、血清生化指标,并观察肠道和肝胰脏的组织病理学,以探讨异育银鲫感染鲤疱疹病毒Ⅱ型(Cy HV-2)的发病机制。结果表明:IH组和I组鲫除淋巴细胞百分比显著升高外(P<0.05),中性粒细胞和单核细胞百分比、红细胞和血栓细胞数、血红蛋白、血栓细胞压积均显著低于H组(P<0.05);I组淋巴细胞百分比显著高于IH组(P<0.05),而中性粒细胞百分比却显著低于IH组(P<0.05),其他各项指标无显著差异。IH组和I组除天门冬氨酸基转移酶/丙氨酸氨基转移酶、白蛋白、白球比例比H组显著升高外(P<0.05),胆碱酯酶和碱性磷酸酶活性、总胆汁酸、甘油三酯、高密度脂蛋白/低密度脂蛋白均显著下降(P<0.05)。I组与IH组结果比较,天门冬氨酸基转移酶、天门冬氨酸基转移酶/丙氨酸氨基转移酶、白蛋白、白球比例均出现显著升高(P<0.05),而胆碱酯酶、碱性磷酸酶出现显著下降(P<0.05),总胆汁酸含量下降了38倍,下降明显,其他均无显著变化。扫描电镜观察发现,H组红细胞表面光滑完整,IH和I组均有一定的损伤;相比H组,IH组、I组的肝胰脏和肠道的组织结构损伤,且随着患病严重性的增加而加重。
Three types of allogyogenetics silver crucian carp（ Carassius auratus gibelio） samples were collected from ponds of never Cyprinid herpesvirusⅡpathogenesis（ Group H, virus carriers）, from ponds of being early stage of the disease（ Group IH） and from ponds of being later stage of the disease（ Group I） in Dafeng city, Jiangsu province. Variation in blood biochemical indices andhistopathology was studied in allogyogenetics silver crucian carp to make insight of the variation in blood components and functions and the pathogenesis. There were significant decrease in neutrophilic percentage, monocyte percentage, erythrocyte count, hemoglobin level, platelet count, and platelet hematocrit in Groups IH and I than those in Group H, except for lymphocyte percentage（ P〈0.05）.Compared with the IH group, lymphocytes percentage was significantly increased in Group I（ P〈0.05）, while neutrophils percentage was significantly decreased（ P〈0.05）, without significant differences in other indices in the three groups. The albumin（ ALB） /globulin（ GLB） ratio and activities of aspartate aminotransferase（ AST） and alanine aminotransferase（ ALT） were found to be significantly higher in Groups IH and I（ P〈0.05）, while activities of ache（ CHE）, alkaline phosphatase（ ALP）, levels of total bile acid（ TBA）, whose content was decreased by 38 times, and triglyceride（ TG）, and high density lipoprotein（ HDL） /low density lipoprotein（ LDL） ratio were significantly decreased（ P〈0.05）, without significant differences in other indices in the three groups. Scanning electron microscope observation revealed that the erythrocytes in Group H had smooth and complete surface, that the erythrocyte in Groups IH and I had some injuries. Compared with Group H, hepatopancreas and intestine in Groups IH and I were structurally damaged, the severity being increased with the increase in the prevalence of the disease.
出处
《水产学杂志》
CAS
2016年第2期16-23,共8页
Chinese Journal of Fisheries
基金
江苏省水产三新工程项目(D2015-12)
关键词
鲤疱疹病毒Ⅱ型
异育银鲫
血液成分
生化指标
组织病理
Cyprinid herpesvirus 2（Cy HV-2）
allogyogenetics silver crucian carp（Carassius auratus gibelio）
blood component
biochemical indicator
histopathology
