摘要
目的:建立HPLC-ELSD同时测定枇杷叶中6种三萜酸成分含量的方法。方法:采用Ultimate XB C18色谱柱(4.6mm×250 mm,5μm),流动相乙腈-甲醇-0.5%醋酸铵溶液,梯度洗脱,流速1.0 mL·min-1,柱温35℃,蒸发光散射检测器漂移管温度95℃,氮气流速2.5 L·min-1。结果:蔷薇酸、委陵菜酸、马斯里酸、科罗索酸、齐墩果酸、熊果酸分别在0.436～2.180,0.392～1.960,0.39～1.960,0.856～4.280,0.424～2.120,1.592～7.960μg,进样质量对数值与峰面积对数值呈良好线性关系;平均回收率分别为98.9%(RSD 3.8%),96.3%(RSD 2.7%),97.7%(RSD 2.6%),97.6%(RSD 3.2%),96.6%(RSD1.9%),102.5%(RSD 2.5%)。结论:建立的含量测定方法简便、准确、分离效果好、重复性好,为更好地控制枇杷叶药材质量提供试验依据。
Objective： To establish an HPLC-ELSD method for simultaneous quantitation of six triterpene acids from Eriobotrya japonica. Method： Samples were analyzed with Ultimate XB C18 column （4.6 mm × 250 mm, 5 μm） and eluted with acetonitrile-methanol-0.5 % ammonium acetate solution as mobile phases in gradient mode, flow rate was 1.0 mL·min^-1, column temperature of 35 ℃ , drift tube temperature of ELSD was set at 95 ℃ , and flow rate of nitrogen was 2.5 L· min ^-1 Result： There were good linear relationship between natural logarithm of peak areas and injection quality at the range of 0. 436-2. 180, 0. 392-1. 960, 0.39-1. 960, 0. 856- 4.280, 0.424-2. 120, 1.592-7.960 μg for rosolic acid, tormentic acid, maslinic acid, corosolic acid, oleanolic acid, ursolic acid, respectively. The average recoveries of these six triterpene acids were 98.9% （RSD 3.8%）, 96.3% （RSD 2.7%）, 97.7% （RSD 2.6%）, 97.6% （RSD 3.2%）, 96.6% （RSD 1.9%）, 102.5% （RSD 2.5%）, respectively. Conclusion： This established method was simple, accurate and repeatable with good separation, it could provide experimental basis for a better quality control of E. japonica.
出处
《中国实验方剂学杂志》
CAS
北大核心
2013年第1期84-87,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
江苏省产学研前瞻性联合研究项目(BY2009144)
关键词
枇杷叶
蔷薇酸
委陵菜酸
马斯里酸
科罗索酸
齐墩果酸
熊果酸
Eriobotrya japonica
rosolic acid
tormentic acid
maslinic acid
corosolic acid
oleanolicacid
ursolic acid
