摘要
采用反相高效液相色谱法测定了枇杷叶中科罗索酸的含量。将样品在90%乙醇溶液中于90℃下提取3.0h,提取3次,浓缩溶液后加水沉淀,将沉淀溶解于甲醇溶液后在反相色谱柱上分离,以甲醇-1.0%醋酸水溶液(体积比为88∶12)为流动相,流速0.8m L/m in,在波长215nm处进行测定。结果表明:安徽黄山产枇杷叶中科罗索酸含量(以质量分数计)为0.36%,科罗索酸平均加标回收率为99%。该方法方便快速,结果准确可靠。
Corosolic acid is clinically proven to activate cell glucose-transporter ＂shuttles＂ and thus helps balance blood glucose levels. A method was developed for the determination of corosolic acid in Eriobotrya japonica leaves by reversed-phase high performance liquid chromatography （RP-HPLC）. The peak of corosolic acid in Eriobotrya japonica leaves was qualitatively analyzed by comparing the retention times and mass spectra of corosolic acid standard and the extract on HPLC-mass spectrometry （MS）. Eriobotrya japonica leaves were extracted thrice for 3.0 h at 90 ℃ with 90% ethanol. The extract was concentrated and deposited by water to remove the impurity which interfered the determination. The deposit was dissolved by methanol and separated on an ODS column （250 mm × 4.5 mm i. d. , 5 μm）. Methanol-1.0% acetic acid （88： 12, v/v） was used as the mobile phase with a flow rate of 0. 8 mL/min. The detection wavelength was 215 nm. The linearity was good within the range of 1.0 -5.0 μg （r = 0.999 9 ）. The corosolic acid content of Eriobotrya japonica leaves from Huangshan was 0.35%. The average recovery of corosolic acid was 99%. The method is simple, rapid, accurate and reliable for the determination of corosolic acid in Eriobotrya japonica leaves.
出处
《色谱》
CAS
CSCD
北大核心
2006年第5期492-494,共3页
Chinese Journal of Chromatography
基金
国家星火计划项目(2004EA710012)
关键词
反相高效液相色谱法
科罗索酸
枇杷叶
reversed-phase high performance liquid chromatography （RP-HPLC）
corosolicacid
Eriobotrya japonica leaves
