摘要
从四川红原地区土壤中分离纯化得到对重金属铜离子(Cu2+)具有良好抗性的菌株YS-22,经16S rDNA序列比对鉴定该菌为克雷伯氏菌属(Klebsiella)中的产酸克雷伯氏菌(K.oxytoca).利用该菌进行生物吸附研究,结果显示其对水体中的重金属Cu2+具有良好的吸附性能,吸附行为能很好地符合pseudo二级动力学模型和Langmuir等温吸附模型,不同的培养条件以及菌体所处的不同生长时期会影响菌体的吸附效果,PGY培养基培养达到生长指数期时菌体用于吸附效果最好.最佳吸附pH值为5.30,吸附迅速,约60 min即可达到吸附平衡,最大单位吸附量达到117.6mg g-1干重菌体.研究表明该菌株作为一种生物吸附剂用于去除废水中的重金属铜污染具有一定的实际应用价值.
Biosorption is a kind of effective and economical method to remove heavy metals from polluted waters. A bacterial strain YS-22 with high resistance to Cu2＋, was isolated from soil samples collected from Hongyuan, Sichuan and was investigated. Strain YS-22 was identified as Klebsiella oxytoca according to its high 16SrDNA sequence similarity （〉99%） to K. oxytoca. The maximum adsorption capacity of strain YS-22 for Cu2＋ was 117.6 mg g~ at pH 5.30. Adsorption process was very fast initially and reached equilibrium in about 60 minutes following pseudo second order rate kinetics and Langmuir adsorption isotherm models. In conclusion, the strain YS-22 can be use as an effective biological adsorbent to treat Cu2＋ wastewater.
出处
《应用与环境生物学报》
CAS
CSCD
北大核心
2012年第6期964-970,共7页
Chinese Journal of Applied and Environmental Biology
基金
国家自然科学基金项目(Nos.31000070
51074149)资助~~
