摘要
通过对电子垃圾污染环境来源的样品利用选择性培养基进行微生物培养与纯化,分离、筛选出12株对重金属铜(Cu2+)具有较高抗性的菌株;其中,菌株HZB同时对铬(Cr2+)和镉(Cd2+)也具有较高的抗性.通过对菌株HZB的生理生化特性及16S r DNA序列进行分析,结果表明菌株HZB属于蜡状芽孢杆菌,它在中性环境中生长良好.利用重金属最低抑制浓度的检测,发现菌株HZB能单抗4.2 mmol/L的Cr2+、2.5 mmol/L的Cu2+和0.7 mmol/L的Cd2+.在Cu2+的环境中,菌株HZB对Cu2+具有明显的吸附作用;并且在应激和适应的状态下,菌株HZB显示出相似的吸附曲线.曲线拟合结果表明,在适应的状态下,HZB菌体对Cu2+的吸附更符合Langmuir吸附理论.
Twelve strains with a high resistance to heavy metal(Cu2+)were isolated from the electron-ic-waste sample by selective mediums. Strain HZB, one of the 12 bacteria, exhibited a high resistance to Cr2+and Cd2+, too. It performed the resistance against 4.2 mmol/L of Cr2+, 2.5 mmol/L of Cu2+and 0.7 mmol/L of Cd2+, respectively. The results from physiological-biochemical characteristics and 16S rDNA sequence indicated that strain HZB belonged to Bacillus cereus. Under the pressure of copper, strain HZB had an obvious capacity of copper adsorption, and showed similar adsorption curve under the condition of stress and adaptation. The bio-sorption of HZB toward Cu2+at adaptation of state was in accordance with the Langmuir model.
出处
《韩山师范学院学报》
2016年第3期78-83,共6页
Journal of Hanshan Normal University
基金
广东省科技计划项目(项目编号:2012B060400016)
关键词
重金属
抗性菌株
铜离子
电子垃圾
heavy metal
resistant strain
Cu2+
electronic-waste
