摘要
目的性激素和促性腺激素对卵巢癌的影响一直存在争议,文中研究性激素和促性腺激素对卵巢癌细胞株的体外生长的调控,并探讨可能机制,为进一步的临床研究提供理论依据。方法将不同浓度雌激素、孕激素、人绒毛促性腺生长激素(human chorionic gonadotropin,hCG)作用于卵巢癌细胞株SKOV3,利用四甲基偶氮唑兰(MTT)比色法检测细胞在24、48、72h的增殖情况,流式细胞仪检测用药后细胞凋亡率及细胞周期的改变,实时荧光定量PCR检测用药前后各组细胞表皮生长因子受体(epidermal growth factor receptor,EGFR)、Bcl-2、Bax mRNA的表达量。结果孕激素和浓度为10-4mol/L的雌激素抑制细胞的生长,诱导细胞凋亡,作用细胞72h其EGFR、Bcl-2 mRNA表达量显著下调;10-4mol/L的雌激素作用72 h后细胞周期分布以S期为主,孕激素作用72 h后大量细胞阻滞在G0/G1期;hCG和浓度小于10-6mol/L的雌激素促进细胞增殖,作用细胞72 h其EGFR、Bcl-2 mRNA表达量均上调显著,细胞周期分布G2/M期增多。结论孕激素和高浓度的雌激素能抑制卵巢癌细胞的增殖,其机制可能是通过将细胞分别阻滞于G0/G1、S期,下调EGFR和Bcl-2的表达而诱导细胞凋亡来实现的。HCG和低浓度的雌激素能刺激卵巢癌细胞的增殖,其机制可能是通过上调EGFR和Bcl-2的表达而抑制细胞凋亡来实现的。
Objective The hypothesis that reproductive hormones and gonadotrophins may be involved in the development of ovarian cancer is still controversial. This study is to observe the hormone effects and possible mechanism, and to provide theoretical foundation for further clinical researchers. Methods Cells of ovarian cancer line SKOV3 were treated by different doses of estrogen （E）, progesterone （P） and human chorionic gonadotropin （hCG）, then the cell proliferations were measured by 3-（4,5-dimethyhhia- zol-z-yl） -2,5-dipheny tetrazolium blue （MTF） colorimetric assay after 24, 48 and 72 h. After 72 h of treatment, the changes of cell cycle and apoptotic percentage were detected by flow cytometry, and the expressions of EGFR, Bcl-2 and Bax mRNA were detected by real-time PCR. Results P or E at 10^-4 mol/L inhibited cell growth, induced apoptosis significantly and suppressed EGFR and Bcl-2 mRNA expressions. After 72 h treatment at 10^-4moL/L, the percentage of cells in S and G2/M phase of cell cycle raised with E, while that in G0/G1 phase raised with P. Treated with hCG or E at 10^-6 mol/L, cell proliferation increased significantly, after 72 h incuba- tion, the expressions of EGFR and Bcl-2 mRNA increased, and the cell percentage in G2/M phase of cell cycle increased slightly. Conclusion P or high concentration of E could inhibit the proliferation of ovarian cancer cell, the effects were achieved by apoptosis relating to the blocking of cell cycle at G0/G1 and S phase and the down-regulation of the expressions of EGFR and Bcl-2 ; hCG and lower concentration of E could stimulate the proliferation of ovarian cancer cells, the effects were achieved by up-regulation of the ex- pressions of EGFR and Bcl-2.
出处
《医学研究生学报》
CAS
北大核心
2012年第12期1238-1241,共4页
Journal of Medical Postgraduates
