摘要
目的 :构建变形链球菌表面蛋白基因 (pac)唾液粘附区的植物表达质粒pROP1和pRPB1。方法 :用PCR扩增的含变形链球菌表面蛋白唾液粘附区P1片段 (184- 1946bp)与植物的高效表达质粒pROKCP结合 ,构建pROP1表达质粒 ;且将此质粒与Bar基因 (抗除草剂基因 )连接 ,构建表达质粒pRPB1,酶切电泳检测。结果 :从pPC41中扩增的P1片段整合到pROKCP的适当部位 ,构成重组质粒pROP1,从pPBar分离出的Bar基因整合到pROP1,构成重组质粒pRPB1。结论 :本实验成功地构建了携带变形链球菌表面蛋白唾液粘附区的植物表达质粒pROP1和pRPB1。
Objective: Construction of plant expressional plasmid pROP1 and pRPB1 of saliva-binding region(SBR) of surface protein antigen gene of Streptococcus mutans. Methods: We linked P1 gene including SBR with plant expressional plasmid pROKCP to construct pROP1 plasmid, then insert Bar gene into the plasmid and formed pRPB1 plasmid , which is tested by agar electrophoresis after the effect of restriction enzyme. Results: P1 gene was linked to pROKCP plasmid and formed recombinant plasmid pROP1; Bar gene from pPBar plasmid was linked to pROP1 plasmid and formed recombinant plasmid pRPB1. Conclusion: We have successfully constructed plant expressional vector pROP1 and pRPB1 of saliva-binding region. [
出处
《口腔医学纵横》
CSCD
2000年第2期94-96,共3页
Journal of Comprehensive Stomatology
关键词
植物表达质粒
唾液粘附区
龋齿
pac蛋白
plant expressional plasmid
surface protein gene
saliva-binding region
